Potential anticancer effect of aspirin and 2'‑hydroxy‑2,3,5'‑trimethoxychalcone‑linked polymeric micelles against cervical cancer through apoptosis

Lee,Kang Pa; Baek,Suji; Yoon,Myeong Sik; Park,Ji Soo; Hong,Bok Sil; Lee,Sang Ju; Oh,Seung Jun; Kwon,Seung Hae; Lee,Ruda; Lee,Dae Ho; Park,Kang-Seo; Moon,Byung Seok

doi:10.3892/ol.2021.13149

Potential anticancer effect of aspirin and 2'‑hydroxy‑2,3,5'‑trimethoxychalcone‑linked polymeric micelles against cervical cancer through apoptosis

Authors:
- Kang Pa Lee
- Suji Baek
- Myeong Sik Yoon
- Ji Soo Park
- Bok Sil Hong
- Sang Ju Lee
- Seung Jun Oh
- Seung Hae Kwon
- Ruda Lee
- Dae Ho Lee
- Kang-Seo Park
- Byung Seok Moon
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Affiliations: Research and Development Center, UMUST R&D Corporation, Seoul 01411, Republic of Korea, Department of Pharmaceutical Engineering, Hoseo University, Cheonan, Chungnam 31499, Republic of Korea, Department of Nursing, Cheju Halla University, Jeju 63092, Republic of Korea, Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea, Seoul Center, Korean Basic Science Institute, Seoul 02841, Republic of Korea, International Research Organization for Advanced Science and Technology, Kumamoto University, Kumamoto, Japan, Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea, Department of Nuclear Medicine, Ewha Womans University College of Medicine, Seoul 07804, Republic of Korea
Published online on: November 25, 2021 https://doi.org/10.3892/ol.2021.13149
Article Number: 31
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Although early diagnosis and treatment of cancers in women are achievable through continuous diagnostic tests, cervical cancer (CVC) still has a high mortality rate. In the present study, we investigated whether certain nanoparticles (NPs), comprising aspirin conjugated 2'‑hydroxy‑2,3,5'‑trimethoxychalcone chemicals, could induce the apoptosis of cancer cells. HeLa cells were treated with NPs and the cell viability was evaluated using WST‑1 assay. Protein expression of Ki‑67 was measured using immunocytochemistry. In addition, the apoptotic effect of NPs was determined using TUNEL assay. To investigate the apoptosis signaling pathways, reverse transcription quantitative PCR was performed and lipid accumulation was observed via holotomographic microscopy. The IC₅₀ value of the NPs was 4.172 µM in HeLa cells. Furthermore, 10 µM NPs significantly inhibited the cell proliferation and stimulated the apoptosis of HeLa cells. In addition, apoptosis and mitochondrial dysfunction were induced by the NPs through lipid accumulation in HeLa cells, leading to apoptotic signaling cascades. Taken together, the results from the present study demonstrated that the NPs developed promoted apoptosis though efficient lipid accumulation in HeLa cells, suggesting that they may provide a novel way to improve the efficacy of CVC anticancer treatment.

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