Identification of two downregulated circRNAs in patients with acute B‑lymphocytic leukemia

Zhou,Bo; Zhong,Liansheng; Tian,Liu; Zhang,Ye; Wang,Runan; He,Qun; Zhao,Yujie

doi:10.3892/ol.2021.13158

Identification of two downregulated circRNAs in patients with acute B‑lymphocytic leukemia

Authors:
- Bo Zhou
- Liansheng Zhong
- Liu Tian
- Ye Zhang
- Runan Wang
- Qun He
- Yujie Zhao
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Affiliations: Key Laboratory of Cell Biology of Ministry of Public Health, College of Life Sciences, China Medical University, Shenyang, Liaoning 110122, P.R. China, Department of Bioinformatics, College of Life Sciences, China Medical University, Shenyang, Liaoning 110122, P.R. China, Psychiatry and Mental Health Center, Shenyang Mental Health Center, Shenyang, Liaoning 110122, P.R. China, Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110122, P.R. China
Published online on: December 3, 2021 https://doi.org/10.3892/ol.2021.13158
Article Number: 40
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute B‑lymphocytic leukemia (B‑ALL) is associated with a high mortality rate, with no effective treatment strategies available. The identification of diagnostic and prognostic biomarkers of B‑ALL can contribute to the development of novel therapeutic methods and drugs, which can improve the survival outcomes of patients with B‑ALL. The present study aimed to identify downregulated circular RNAs (circRNAs) in patients with B‑ALL. RNA sequencing was performed to construct the circRNA expression profiles in B‑ALL cells and normal human lymphoblasts. The Database for Annotation, Visualization and Integrated Discovery was used to perform Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses. In addition, reverse transcription‑quantitative (RT‑q)PCR analysis was performed to detect the expression levels of the downregulated circRNAs. A total of 263 differentially expressed circRNAs were identified, including 76 upregulated and 187 downregulated circRNAs, respectively. The upregulated circRNAs were mainly enriched in ‘macromolecule modification’, ‘protein modification’ and ‘cellular protein modification processes’, while the downregulated circRNAs were mainly enriched in the ‘negative regulation of RNA biosynthetic processes’, ‘natural killer cell‑mediated cytotoxicity’ and ‘viral carcinogenesis’. RT‑qPCR analysis demonstrated that two of the downregulated circRNAs (hsa_circ_0000745 and chr15:87949594‑87966067‑), identified during microarray analysis were also significantly downregulated in Ball‑1 cells and B‑ALL bone marrow samples. Thus, these circRNAs may serve as biomarkers for patients with B‑ALL.

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