Open Access

Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9

  • Authors:
    • Ryutaro Shimizu
    • Takahito Ohira
    • Takuki Yagyu
    • Tetsuya Yumioka
    • Noriya Yamaguchi
    • Hideto Iwamoto
    • Shuichi Morizane
    • Katsuya Hikita
    • Masashi Honda
    • Atsushi Takenaka
    • Hiroyuki Kugoh
  • View Affiliations

  • Published online on: January 27, 2022     https://doi.org/10.3892/ol.2022.13212
  • Article Number: 92
  • Copyright: © Shimizu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bladder cancer is divided into two molecular subtypes, luminal and basal, which form papillary and nodular tumors, respectively, and are identifiable by gene expression profiling. Although loss of heterozygosity (LOH) of the long arm of human chromosome 9 (9q) has been observed in the early development of both types of bladder cancer, the functional significance of LOH remains to be clarified. The present study introduced human chromosome 9q into basal bladder cancer cell line, SCaBER, using microcell‑mediated chromosome transfer to investigate the effect of LOH of 9q on molecular bladder cancer subtypes. These cells demonstrated decreased proliferation and migration capacity compared with parental and control cells. Conversely, transfer of human chromosome 4 did not change the cell phenotype. Expression level of peroxisome proliferator‑activated receptor (PPAR)γ, a marker of luminal type, increased 3.0‑4.4 fold in SCaBER cells altered with 9q compared with parental SCaBER cells. Furthermore, the expression levels of tumor suppressor PTEN, which regulates PPARγ, also increased in 9q‑altered cells. These results suggested that human chromosome 9q may carry regulatory genes for PPARγ that are involved in the progression of neoplastic transformation of bladder cancer.
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March-2022
Volume 23 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Shimizu R, Ohira T, Yagyu T, Yumioka T, Yamaguchi N, Iwamoto H, Morizane S, Hikita K, Honda M, Takenaka A, Takenaka A, et al: Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9. Oncol Lett 23: 92, 2022.
APA
Shimizu, R., Ohira, T., Yagyu, T., Yumioka, T., Yamaguchi, N., Iwamoto, H. ... Kugoh, H. (2022). Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9. Oncology Letters, 23, 92. https://doi.org/10.3892/ol.2022.13212
MLA
Shimizu, R., Ohira, T., Yagyu, T., Yumioka, T., Yamaguchi, N., Iwamoto, H., Morizane, S., Hikita, K., Honda, M., Takenaka, A., Kugoh, H."Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9". Oncology Letters 23.3 (2022): 92.
Chicago
Shimizu, R., Ohira, T., Yagyu, T., Yumioka, T., Yamaguchi, N., Iwamoto, H., Morizane, S., Hikita, K., Honda, M., Takenaka, A., Kugoh, H."Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9". Oncology Letters 23, no. 3 (2022): 92. https://doi.org/10.3892/ol.2022.13212