Open Access

Biological response of adrenal carcinoma and melanoma cells to mitotane treatment

  • Authors:
    • Ewelina Stelcer
    • Hanna Komarowska
    • Karol Jopek
    • Agnieszka Żok
    • Dariusz Iżycki
    • Agnieszka Malińska
    • Beata Szczepaniak
    • Zhanat Komekbai
    • Marek Karczewski
    • Tomasz Wierzbicki
    • Wiktoria Maria Suchorska
    • Marek Ruchała
    • Marcin Ruciński
  • View Affiliations

  • Published online on: February 10, 2022     https://doi.org/10.3892/ol.2022.13240
  • Article Number: 120
  • Copyright: © Stelcer et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A previous case report described an adrenal incidentaloma initially misdiagnosed as adrenocortical carcinoma (ACC), which was treated with mitotane. The final diagnosis was metastatic melanoma of unknown primary origin. However, the patient developed rapid disease progression after mitotane withdrawal, suggesting a protective role for mitotane in a non‑adrenal‑derived tumor. The aim of the present study was to determine the biological response of primary melanoma cells obtained from that patient, and that of other established melanoma and ACC cell lines, to mitotane treatment using a proliferation assay, flow cytometry, quantitative PCR and microarrays. Although mitotane inhibited the proliferation of both ACC and melanoma cells, its role in melanoma treatment appears to be limited. Flow cytometry analysis and transcriptomic studies indicated that the ACC cell line was highly responsive to mitotane treatment, while the primary melanoma cells showed a moderate response in vitro. Mitotane modified the activity of several key biological processes, including ‘mitotic nuclear division’, ‘DNA repair’, ‘angiogenesis’ and ‘negative regulation of ERK1 and ERK2 cascade’. Mitotane administration led to elevated levels of DNA double‑strand breaks, necrosis and apoptosis. The present study provides a comprehensive insight into the biological response of mitotane‑treated cells at the molecular level. Notably, the present findings offer new knowledge on the effects of mitotane on ACC and melanoma cells.
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April-2022
Volume 23 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Stelcer E, Komarowska H, Jopek K, Żok A, Iżycki D, Malińska A, Szczepaniak B, Komekbai Z, Karczewski M, Wierzbicki T, Wierzbicki T, et al: Biological response of adrenal carcinoma and melanoma cells to mitotane treatment. Oncol Lett 23: 120, 2022
APA
Stelcer, E., Komarowska, H., Jopek, K., Żok, A., Iżycki, D., Malińska, A. ... Ruciński, M. (2022). Biological response of adrenal carcinoma and melanoma cells to mitotane treatment. Oncology Letters, 23, 120. https://doi.org/10.3892/ol.2022.13240
MLA
Stelcer, E., Komarowska, H., Jopek, K., Żok, A., Iżycki, D., Malińska, A., Szczepaniak, B., Komekbai, Z., Karczewski, M., Wierzbicki, T., Suchorska, W. M., Ruchała, M., Ruciński, M."Biological response of adrenal carcinoma and melanoma cells to mitotane treatment". Oncology Letters 23.4 (2022): 120.
Chicago
Stelcer, E., Komarowska, H., Jopek, K., Żok, A., Iżycki, D., Malińska, A., Szczepaniak, B., Komekbai, Z., Karczewski, M., Wierzbicki, T., Suchorska, W. M., Ruchała, M., Ruciński, M."Biological response of adrenal carcinoma and melanoma cells to mitotane treatment". Oncology Letters 23, no. 4 (2022): 120. https://doi.org/10.3892/ol.2022.13240