miR‑148a promotes cell sensitivity through downregulating SOS2 in radiation‑resistant non‑small cell lung cancer cells

Zhang,Yan; Hu,Xiaoqian

doi:10.3892/ol.2022.13255

miR‑148a promotes cell sensitivity through downregulating SOS2 in radiation‑resistant non‑small cell lung cancer cells

Authors:
- Yan Zhang
- Xiaoqian Hu
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Affiliations: Department of Laboratory, Xingtai People's Hospital, Xingtai, Hebei 054001, P.R. China
Published online on: February 24, 2022 https://doi.org/10.3892/ol.2022.13255
Article Number: 135
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Non‑small cell lung carcinoma (NSCLC) is the most common type of lung cancer; however, radioresistance is a significant barrier in NSCLC radiotherapy. MicroRNA (miR)‑148a has been reported to be a tumor suppressor in various types of cancer, including NSCLC. In the present study, the potential role of miR‑148a in regulating radiosensitivity of NSCLC cells was investigated. Serum miR‑148a expression was evaluated by reverse transcription‑quantitative PCR in patients with NSCLC and healthy controls. The effects of miR‑148a on cell viability, migration and invasion were assessed by Cell Counting Kit‑8 and Transwell assays in radiation‑resistant NSCLC cells. Serum miR‑148a was downregulated in patients with NSCLC compared with healthy controls and its expression was significantly increased after radiotherapy. By contrast, miR‑148a expression was decreased in the radioresistant patients compared with the radiosensitivity patients. Additionally, miR‑148a overexpression inhibited the cell proliferation, migration and invasion of radiation‑resistant NSCLC cells. In addition, miR‑148a had putative binding site with Son of Sevenless 2 (SOS2) and negatively regulated SOS2 expression. Silencing SOS2 expression significantly suppressed miR‑148a inhibitor‑induced increase in radiosensitivity in NSCLC. In conclusion, the results of the present study suggested that miR‑148a could enhance the radiosensitivity of NSCLC cells through targeting SOS2, thus providing potential therapeutic targets to improve radiotherapy in NSCLC.

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