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miR‑4735‑3p inhibits cell migration and invasion of gastric cancer by downregulating NEDD9

  • Authors:
    • Yanchun Ma

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  • Published online on: June 10, 2022     https://doi.org/10.3892/ol.2022.13373
  • Article Number: 253

  • Copyright: © Ma . This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) comprises the 3rd cause of cancer‑related death worldwide. Increased expression of neural precursor cell expressed, developmentally downregulated 9 (NEDD9) is commonly observed in GC, however, its underlying molecular mechanism in GC remains unknown. The potential interaction between miR‑4735‑3p and NEDD9 was predicted by TargetScan 7.1. Expression profiles of miR‑4735‑3p and NEDD9 were examined between GC tissues and normal tissues by reverse transcription‑quantitative (RT‑q) PCR. The relationship between miR‑4735‑3p and NEDD9 was validated by RT‑qPCR, western blotting, dual luciferase reporter assay and RNA immunoprecipitation assay. Biological relationship between miR‑4735‑3p and NEDD9 was evidenced by the cell invasion and cell migration assays. NEDD9 level was negatively associated with miR‑4735‑3p level in GC tissues. miR‑4735‑3p suppressed NEDD9 levels in GC cells. NEDD9 was revealed to be a target gene of miR‑4735‑3p. miR‑4735‑3p overexpression suppressed cell migration and invasion of GC cells, which were antagonized by overexpression of NEDD9. Moreover, miR‑4735‑3p mimic decreased the levels of matrix metalloproteinases 2/9, increased the level of E‑cadherin, which were reversed by overexpression of NEDD9. Collectively, the present study provided a potential mechanism for the tumor suppressor role of miR‑4735‑3p in GC by targeting NEDD9.
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August-2022
Volume 24 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ma Y: miR‑4735‑3p inhibits cell migration and invasion of gastric cancer by downregulating NEDD9. Oncol Lett 24: 253, 2022.
APA
Ma, Y. (2022). miR‑4735‑3p inhibits cell migration and invasion of gastric cancer by downregulating NEDD9. Oncology Letters, 24, 253. https://doi.org/10.3892/ol.2022.13373
MLA
Ma, Y."miR‑4735‑3p inhibits cell migration and invasion of gastric cancer by downregulating NEDD9". Oncology Letters 24.2 (2022): 253.
Chicago
Ma, Y."miR‑4735‑3p inhibits cell migration and invasion of gastric cancer by downregulating NEDD9". Oncology Letters 24, no. 2 (2022): 253. https://doi.org/10.3892/ol.2022.13373