Lidocaine inhibits the proliferation and migration of endometrial cancer cells, and promotes apoptosis by inducing autophagy

Long,Dingde; Chen,Yayu; Qu,Liangchao; Dong,Yang

doi:10.3892/ol.2022.13467

Lidocaine inhibits the proliferation and migration of endometrial cancer cells, and promotes apoptosis by inducing autophagy

Authors:
- Dingde Long
- Yayu Chen
- Liangchao Qu
- Yang Dong
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Affiliations: Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China, Department of Anesthesiology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China
Published online on: August 17, 2022 https://doi.org/10.3892/ol.2022.13467
Article Number: 347
Copyright: © Long et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

As a gynecological malignancy, endometrial cancer (EC) has a high incidence and mortality rate in women. The aim of the present study was to investigate the mechanism of EC and to identify novel effective treatment methods for this disease. The viability and proliferation of the RL95‑2 human endometrial cancer cell line were assessed using Cell Counting Kit‑8 assays. Colony formation, wound healing, Transwell, TUNEL and immunofluorescence assays were used to assess the effects of 5, 10 and 15 mM lidocaine on the colony formation, migration, invasiveness, apoptosis and Beclin 1 protein expression of RL95‑2 cells, respectively. Furthermore, western blotting was used to analyze the protein expression levels of apoptosis‑ and autophagy‑related proteins. The results demonstrated that lidocaine inhibited the viability, proliferation and migration of EC cells, and promoted apoptosis. Furthermore, lidocaine was demonstrated to induce autophagy and Beclin 1 protein expression in EC cells. In conclusion, lidocaine inhibited the proliferation and migration of EC cells, and promoted apoptosis via autophagy induction, which indicated that lidocaine may be a potential therapeutic drug for the treatment of EC.

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