Association between circ_0004365 and cisplatin resistance in esophageal squamous cell carcinoma

Yamada,Moyuru; Tanaka,Koji; Yamamoto,Kenichi; Matsumoto,Hisatake; Yamasaki,Makoto; Yamashita,Kotaro; Makino,Tomoki; Saito,Takuro; Yamamoto,Kazuyoshi; Takahashi,Tsuyoshi; Kurokawa,Yukinori; Nakajima,Kiyokazu; Okada,Yukinori; Eguchi,Hidetoshi; Doki,Yuichiro

doi:10.3892/ol.2023.14054

Association between circ_0004365 and cisplatin resistance in esophageal squamous cell carcinoma

Authors:
- Moyuru Yamada
- Koji Tanaka
- Kenichi Yamamoto
- Hisatake Matsumoto
- Makoto Yamasaki
- Kotaro Yamashita
- Tomoki Makino
- Takuro Saito
- Kazuyoshi Yamamoto
- Tsuyoshi Takahashi
- Yukinori Kurokawa
- Kiyokazu Nakajima
- Yukinori Okada
- Hidetoshi Eguchi
- Yuichiro Doki
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Affiliations: Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan, Department of Statistical Genetics, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan, Department of Traumatology and Acute Critical Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565‑0871, Japan
Published online on: September 18, 2023 https://doi.org/10.3892/ol.2023.14054
Article Number: 467
Copyright: © Yamada et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cisplatin is one of the most predominant drugs for the chemotherapy of esophageal squamous cell carcinoma (ESCC); however, the underlying resistance mechanisms are still almost unknown. The present study performed RNA sequencing of human circular RNA (circRNA) in TE11 cells and cisplatin‑resistant TE11 cells (TE11R). The expression profiles determined using CIRCexplorer2 revealed that the expression of circ_0004365, mapped on the Semaphorin 3C gene, was significantly greater in TE11R compared with in TE11. In reverse transcription‑quantitative PCR, circ_0004365 expression was observed in human ESCC and non‑tumor tissues and was significantly upregulated in ESCC tumor tissues after chemotherapy. Circ_0004365 expression was significantly upregulated in patients with poor pathological response (P=0.02). Furthermore, patients with advanced pT stage showed an upregulation in circ_0004365 expression after chemotherapy (P=0.02). The MTT assay revealed that knockdown of circ_0003465 in TE11 significantly decreased resistance to cisplatin. In conclusion, the present study suggested that circ_0004365 was associated with cisplatin resistance in ESCC and can be used as both a novel biomarker and a therapeutic target.

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