Open Access

Agrin is a novel oncogenic protein in thyroid cancer

  • Authors:
    • Anna Adamiok-Ostrowska
    • Małgorzata Grzanka
    • Barbara Czarnocka
  • View Affiliations

  • Published online on: September 25, 2023     https://doi.org/10.3892/ol.2023.14070
  • Article Number: 483
  • Copyright: © Adamiok-Ostrowska et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Agrin (AGRN) is a matricellular glycoprotein involved in extracellular signal transduction. AGRN is involved in tumorigenesis and cancer progression; however, the role of AGRN in thyroid cancer (TC) remains unclear. In the present study, using cell lines derived from various subtypes of TC including CGTH, FTC‑133 and BcPAP and transcriptomic data from patients with TC, the role of AGRN in TC was analyzed by migration, invasion, viability and proliferation assays as well as Western blot with EMT markers. AGRN expression was significantly increased in thyroid tumors and cell lines derived from various TC subtypes. The highest AGRN expression was found in follicular and papillary thyroid carcinoma subtypes. Immunocytochemistry revealed nuclear AGRN localization in normal (NTHY) and TC cells. Silencing of AGRN decreased viability, proliferation, migration and invasion of TC cell lines by upregulating vimentin and downregulating N‑cadherin and E‑cadherin. Furthermore, the expression of AGRN was associated with neutrophil infiltration in thyroid tumors. In conclusion, the present results indicated that increased AGRN expression promoted tumorigenic phenotypes of TC cells, while AGRN expression was associated with immune infiltration in thyroid tumors. AGRN may represent a target for future cancer therapy and requires further evaluation.
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November-2023
Volume 26 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Adamiok-Ostrowska A, Grzanka M and Czarnocka B: Agrin is a novel oncogenic protein in thyroid cancer. Oncol Lett 26: 483, 2023.
APA
Adamiok-Ostrowska, A., Grzanka, M., & Czarnocka, B. (2023). Agrin is a novel oncogenic protein in thyroid cancer. Oncology Letters, 26, 483. https://doi.org/10.3892/ol.2023.14070
MLA
Adamiok-Ostrowska, A., Grzanka, M., Czarnocka, B."Agrin is a novel oncogenic protein in thyroid cancer". Oncology Letters 26.5 (2023): 483.
Chicago
Adamiok-Ostrowska, A., Grzanka, M., Czarnocka, B."Agrin is a novel oncogenic protein in thyroid cancer". Oncology Letters 26, no. 5 (2023): 483. https://doi.org/10.3892/ol.2023.14070