Assessment of the expression of microRNAs‑221‑3p, ‑146a‑5p, ‑16‑5p and BCL2 in oncocytic carcinoma of the breast: A case report

Koi,Yumiko; Yamamoto,Yuki; Fukunaga,Saori; Kajitani,Keiko; Ohara,Masahiro; Daimaru,Yutaka; Tahara,Hidetoshi; Tamada,Ryuichiro

doi:10.3892/ol.2023.14123

Assessment of the expression of microRNAs‑221‑3p, ‑146a‑5p, ‑16‑5p and BCL2 in oncocytic carcinoma of the breast: A case report

Authors:
- Yumiko Koi
- Yuki Yamamoto
- Saori Fukunaga
- Keiko Kajitani
- Masahiro Ohara
- Yutaka Daimaru
- Hidetoshi Tahara
- Ryuichiro Tamada
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Affiliations: Department of Breast Oncology, National Hospital Organization Kyushu Cancer Center, Fukuoka 811‑1395, Japan, Department of Cellular and Molecular Biology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734‑8553, Japan, Department of Breast Surgery, Japan Agricultural Co‑operatives Hiroshima General Hospital, Hatsukaichi, Hiroshima 738‑8503, Japan, Section of Pathological Research and Laboratory, Japan Agricultural Co‑operatives Hiroshima General Hospital, Hiroshima 738‑8503, Japan, Department of Surgery, Nishiki Hospital, Yamaguchi 741‑0061, Japan
Published online on: October 31, 2023 https://doi.org/10.3892/ol.2023.14123
Article Number: 535
Copyright: © Koi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oncocytic carcinoma of the breast is rare and its molecular profiles remain poorly understood. MicroRNAs (miRNAs/miRs) have been identified as contributors to carcinogenesis at the post‑transcriptional level; thus, an aberrant expression of miRNAs has attracted attention as a potential biomarker of numerous diseases, including cancer. The present study reports the case of a 76‑year‑old woman diagnosed with oncocytic carcinoma of the breast. Considering the distinctive feature of oncocytic carcinoma of the breast, which is the presence of granular eosinophilic cytoplasm containing numerous mitochondria, the present study hypothesized that the expression of mitochondria‑related miRNAs could be altered in oncocytic carcinomas. Aberrant expression levels of the miRNAs previously reported as mitochondria‑related miRNAs, such as miR‑221‑3p, ‑146a‑5p and ‑16‑5p, were revealed in tissue from specimens of oncocytic carcinoma of the breast, compared with that of a more typical type of invasive ductal carcinoma of the breast. The present study highlights the changes in miRNA expression in oncocytic carcinoma of the breast, suggesting its potential as a biomarker for diagnosis.

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1	Chang A and Harawi SJ: Oncocytes, oncocytosis, and oncocytic tumors. Pathol Annu. 27(Pt 1): 263–304. 1992.PubMed/NCBI
2	Tan PH, Ellis I, Allison K, Brogi E, Fox SB, Lakhani S, Lazar AJ, Morris EA, Sahin A, Salgado R, et al: The 2019 WHO classification of tumours of the breast. Histopathology. 77:181–185. 2020. View Article : Google Scholar : PubMed/NCBI
3	Zaratiegui M, Irvine DV and Martienssen RA: Noncoding RNAs and gene silencing. Cell. 128:763–776. 2007. View Article : Google Scholar : PubMed/NCBI
4	Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI
5	Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI
6	Pasquinelli AE: MicroRNAs and their targets: Recognition, regulation and an emerging reciprocal relationship. Nat Rev Genet. 13:271–282. 2012. View Article : Google Scholar : PubMed/NCBI
7	Chen CZ: MicroRNAs as oncogenes and tumor suppressors. N Engl J Med. 353:1768–1771. 2005. View Article : Google Scholar : PubMed/NCBI
8	Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, Ebert BL, Mak RH, Ferrando AA, et al: MicroRNA expression profiles classify human cancers. Nature. 435:834–838. 2005. View Article : Google Scholar : PubMed/NCBI
9	Weiland M, Gao XH, Zhou L and Mi QS: Small RNAs have a large impact: Circulating microRNAs as biomarkers for human diseases. RNA Biol. 9:850–859. 2012. View Article : Google Scholar : PubMed/NCBI
10	Giuliani A, Prattichizzo F, Micolucci L, Ceriello A, Procopio AD and Rippo MR: Mitochondrial (Dys) function in inflammaging: Do mitomiRs influence the energetic, oxidative, and inflammatory status of senescent cells? Mediators Inflamm. 2017:23090342017. View Article : Google Scholar : PubMed/NCBI
11	Jaquenod De Giusti C, Roman B and Das S: The influence of microRNAs on mitochondrial calcium. Front Physiol. 9:12912018. View Article : Google Scholar : PubMed/NCBI
12	Xu D, Takeshita F, Hino Y, Fukunaga S, Kudo Y, Tamaki A, Matsunaga J, Takahashi RU, Takata T, Shimamoto A, et al: miR-22 represses cancer progression by inducing cellular senescence. J Cell Biol. 193:409–424. 2011. View Article : Google Scholar : PubMed/NCBI
13	Liu D, Li L, Zhang XX, Wan DY, Xi BX, Hu Z, Ding WC, Zhu D, Wang XL, Wang W, et al: IX1 promotes tumor lymphangiogenesis by coordinating TGFβ signals that increase expression of VEGF-C. Cancer Res. 74:5597–5607. 2014. View Article : Google Scholar : PubMed/NCBI
14	Goulding H, Pinder S, Cannon P, Pearson D, Nicholson R, Snead D, Bell J, Elston CW, Robertson JF, Blamey RW, et al: A new immunohistochemical antibody for the assessment of estrogen receptor status on routine formalin-fixed tissue samples. Hum Pathol. 26:291–294. 1995. View Article : Google Scholar : PubMed/NCBI
15	Asa SL: My approach to oncocytic tumours of the thyroid. J Clin Pathol. 57:225–232. 2004. View Article : Google Scholar : PubMed/NCBI
16	Durham JR and Fechner RE: The histologic spectrum of apocrine lesions of the breast. Am J Clin Pathol. 113((suppl_1)): S3–S18. 2000.PubMed/NCBI
17	Ragazzi M, de Biase D, Betts CM, Farnedi A, Ramadan SS, Tallini G, Reis-Filho JS and Eusebi V: Oncocytic carcinoma of the breast: Frequency, morphology and follow-up. Hum Pathol. 42:166–175. 2011. View Article : Google Scholar : PubMed/NCBI
18	Gil-Zamorano J, Martin R, Daimiel L, Richardson K, Giordano E, Nicod N, García-Carrasco B, Soares SM, Iglesias-Gutiérrez E, Lasunción MA, et al: Docosahexaenoic acid modulates the enterocyte Caco-2 cell expression of microRNAs involved in lipid metabolism. J Nutr. 144:575–585. 2014. View Article : Google Scholar : PubMed/NCBI
19	Frørup C, Mirza AH, Yarani R, Nielsen LB, Mathiesen ER, Damm P, Svare J, Engelbrekt C, Størling J, Johannesen J, et al: Plasma exosome-enriched extracellular vesicles from lactating mothers with type 1 diabetes contain aberrant levels of miRNAs during the postpartum period. Front Immunol. 12:7445092021. View Article : Google Scholar : PubMed/NCBI
20	Barutta F, Corbetta B, Bellini S, Guarrera S, Matullo G, Scandella M, Schalkwijk C, Stehouwer CD, Chaturvedi N, Soedamah-Muthu SS, et al: MicroRNA 146a is associated with diabetic complications in type 1 diabetic patients from the EURODIAB PCS. J Transl Med. 19:4752021. View Article : Google Scholar : PubMed/NCBI
21	Alamro H, Bajic V, Macvanin MT, Isenovic ER, Gojobori T, Essack M and Gao X: Type 2 diabetes mellitus and its comorbidity, Alzheimer's disease: Identifying critical microRNA using machine learning. Front Endocrinol (Lausanne). 13:10846562023. View Article : Google Scholar : PubMed/NCBI
22	Bandiera S, Matégot R, Girard M, Demongeot J and Henrion-Caude A: MitomiRs delineating the intracellular localization of microRNAs at mitochondria. Free Radic Biol Med. 64:12–19. 2013. View Article : Google Scholar : PubMed/NCBI
23	Jagannathan R, Thapa D, Nichols CE, Shepherd DL, Stricker JC, Croston TL, Baseler WA, Lewis SE, Martinez I and Hollander JM: Translational regulation of the mitochondrial genome following redistribution of mitochondrial microRNA in the diabetic heart. Circ Cardiovasc Genet. 8:785–802. 2015. View Article : Google Scholar : PubMed/NCBI
24	Jacques C, Guillotin D, Fontaine JF, Franc B, Mirebeau-Prunier D, Fleury A, Malthiery Y and Savagner F: DNA microarray and miRNA analyses reinforce the classification of follicular thyroid tumors. J Clin Endocrinol Metab. 98:E981–E989. 2013. View Article : Google Scholar : PubMed/NCBI
25	Titov SE, Poloz TL, Veryaskina YA and Anishchenko VV: Cytological and molecular diagnosis of Hürthle cell thyroid tumors: Analysis of three cases. Mol Clin Oncol. 15:1492021. View Article : Google Scholar : PubMed/NCBI
26	Di Meo A, Saleeb R, Wala SJ, Khella HW, Ding Q, Zhai H, Krishan K, Krizova A, Gabril M, Evans A, et al: A miRNA-based classification of renal cell carcinoma subtypes by PCR and in situ hybridization. Oncotarget. 9:2092–2104. 2018. View Article : Google Scholar : PubMed/NCBI
27	Si C, Yu Q and Yao Y: Effect of miR-146a-5p on proliferation and metastasis of triple-negative breast cancer via regulation of SOX5. Exp Ther Med. 15:4515–4521. 2018.PubMed/NCBI
28	Chen G, Umelo IA, Lv S, Teugels E, Fostier K, Kronenberger P, Dewaele A, Sadones J, Geers C and De Grève J: miR-146a inhibits cell growth, cell migration and induces apoptosis in non-small cell lung cancer cells. PLoS One. 8:e603172013. View Article : Google Scholar : PubMed/NCBI
29	Zhang C, Zhang J, Zhang A, Wang Y, Han L, You Y, Pu P and Kang C: PUMA is a novel target of miR-221/222 in human epithelial cancers. Int J Oncol. 37:1621–1626. 2010.PubMed/NCBI
30	Rippo MR, Olivieri F, Monsurrò V, Prattichizzo F, Albertini MC and Procopio AD: MitomiRs in human inflamm-aging: A hypothesis involving miR-181a, miR-34a and miR-146a. Exp Gerontol. 56:154–163. 2014. View Article : Google Scholar : PubMed/NCBI
31	Hockenbery D, Nuñez G, Milliman C, Schreiber RD and Korsmeyer SJ: Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature. 348:334–336. 1990. View Article : Google Scholar : PubMed/NCBI
32	Bender CE, Fitzgerald P, Tait SW, Llambi F, McStay GP, Tupper DO, Pellettieri J, Sánchez Alvarado A, Salvesen GS and Green DR: Mitochondrial pathway of apoptosis is ancestral in metazoans. Proc Natl Acad Sci USA. 109:4904–4909. 2012. View Article : Google Scholar : PubMed/NCBI
33	Oltvai ZN, Milliman CL and Korsmeyer SJ: Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 74:609–619. 1993. View Article : Google Scholar : PubMed/NCBI
34	Giuliani A, Cirilli I, Prattichizzo F, Mensà E, Fulgenzi G, Sabbatinelli J, Graciotti L, Olivieri F, Procopio AD, Tiano L and Rippo MR: The mitomiR/Bcl-2 axis affects mitochondrial function and autophagic vacuole formation in senescent endothelial cells. Aging (Albany NY). 10:2855–2873. 2018. View Article : Google Scholar : PubMed/NCBI
35	Cimmino A, Calin GA, Fabbri M, Iorio MV, Ferracin M, Shimizu M, Wojcik SE, Aqeilan RI, Zupo S, Dono M, et al: miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci USA. 102:13944–13949. 2005. View Article : Google Scholar : PubMed/NCBI
36	Patel N, Garikapati KR, Ramaiah MJ, Polavarapu KK, Bhadra U and Bhadra MP: miR-15a/miR-16 induces mitochondrial dependent apoptosis in breast cancer cells by suppressing oncogene BMI1. Life Sci. 164:60–70. 2016. View Article : Google Scholar : PubMed/NCBI
37	Xia L, Zhang D, Du R, Pan Y, Zhao L, Sun S, Hong L, Liu J and Fan D: miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells. Int J Cancer. 123:372–379. 2008. View Article : Google Scholar : PubMed/NCBI
38	Nissen T and Wynn R: The clinical case report: A review of its merits and limitations. BMC Res Notes. 7:2642014. View Article : Google Scholar : PubMed/NCBI