Expression and effect of miR‑27b in primary liver cancer
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- Published online on: December 20, 2023 https://doi.org/10.3892/ol.2023.14198
- Article Number: 65
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Copyright: © Zhuo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
The occurrence and development of primary liver cancer is associated with microRNA. Specifically, the expression of microRNA‑27b (miR‑27b) is upregulated in four liver cancer drug‑resistance cell lines. Despite that, the function of miR‑27b in liver cancer is not clear yet. The aim of the present study was to investigate the effect of miR‑27b expression during oncogenesis, cell proliferation, apoptosis and chemotherapy resistance development in a model of liver cancer. Expression of miR‑27b was detected with reverse transcription‑quantitative PCR. To establish stable overexpression of miR‑27b and negative control liver cancer cell lines, a lentiviral pre‑miR‑27b overexpression vector and negative control vector were transfected into each cell line. Cell Counting Kit‑8 assay, clone formation assay and immunohistochemical assay were used to detect cell proliferation. Apoptosis and drug sensitivity were detected by flow cytometry and MTT assay, respectively. The expression level of miR‑27b in liver cancer tissues was also lower than in liver tissues adjacent to the tumor. Two stable miR‑27b overexpression liver cancer cell lines (Huh‑7/miR‑27b and HepG2/miR‑27b) and their control cell lines (Huh‑7/NC and HepG2/NC) were successfully constructed. It was revealed that upregulation of miR‑27b can suppress cell proliferation, promote cell apoptosis and chemotherapy resistance. In addition, the findings of the present study demonstrated that patients with cirrhosis expressed lower miR‑27b compared with patients without cirrhosis. The expression level of miR‑27b was significantly associated with the age, serum alpha‑fetoprotein and alanine aminotransferase level of patients with liver cancer. Meanwhile, it was indicated that the disease survival time of the low miR‑27b expression group was longer than that of the high miR‑27b expression group. The present study suggested that miR‑27b functions as a liver cancer suppressor. Moreover, miR‑27b can act as a biomarker to estimate drug sensitivity to chemotherapy in patients with liver cancer.
