Open Access

Influence of Zika virus on the cytotoxicity, cell adhesion, apoptosis and inflammatory markers of glioblastoma cells

  • Authors:
    • Daniel Rodrigo Marinowic
    • Gabriele Goulart Zanirati
    • Pamella Nunes Azevedo
    • Ângela Zanatta
    • Ismael Plentz
    • Allan Marinho Alcará
    • Fernanda Bueno Morrone
    • Thamiris Becker Scheffel
    • Angélica Regina Cappellari
    • Paulo Michel Roehe
    • Ana Paula Muterle Varela
    • Denise Cantarelli Machado
    • Fabiana Spillari Viola
    • Jaderson Costa Da Costa
  • View Affiliations

  • Published online on: February 28, 2024     https://doi.org/10.3892/ol.2024.14309
  • Article Number: 176
  • Copyright: © Marinowic et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioblastoma (GBM) is one of the most common types of brain tumor in adults. Despite the availability of treatments for this disease, GBM remains one of the most lethal and difficult types of tumors to treat, and thus, a majority of patients die within 2 years of diagnosis. Infection with Zika virus (ZIKV) inhibits cell proliferation and induces apoptosis, particularly in developing neuronal cells, and thus could potentially be considered an alternative for GBM treatment. In the present study, two GBM cell lines (U‑138 and U‑251) were infected with ZIKV at different multiplicities of infection (0.1, 0.01 and 0.001), and cell viability, migration, adhesion, induction of apoptosis, interleukin levels and CD14/CD73 cell surface marker expression were analyzed. The present study demonstrated that ZIKV infection promoted loss of cell viability and increased apoptosis in U‑138 cells, as measured by MTT and triplex assay, respectively. Changes in cell migration, as determined by wound healing assay, were not observed; however, the GBM cell lines exhibited an increase in cell adhesion when compared with non‑tumoral cells (Vero). The Luminex immunoassay showed a significant increase in the expression levels of IL‑4 specifically in U‑251 cells (MOI 0.001) following exposure to ZIKV. There was no significant change in the expression levels of IFN‑γ upon ZIKV infection in the cell lines tested. Furthermore, a marked increase in the percentage of cells expressing the CD14 surface marker was observed in both GBM cell lines compared with in Vero cells; and significantly increased CD73 expression was observed particularly in U‑251 cells, when compared with uninfected cells. These findings indicate that ZIKV infection could lead to reduced cell viability, elevated CD73 expression, improved cellular adherence, and higher rates of apoptosis in glioblastoma cells. Further studies are required to explore the potential use of ZIKV in the treatment of GBM.
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April-2024
Volume 27 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Marinowic DR, Zanirati GG, Azevedo PN, Zanatta Â, Plentz I, Alcará AM, Morrone FB, Scheffel TB, Cappellari AR, Roehe PM, Roehe PM, et al: Influence of Zika virus on the cytotoxicity, cell adhesion, apoptosis and inflammatory markers of glioblastoma cells. Oncol Lett 27: 176, 2024.
APA
Marinowic, D.R., Zanirati, G.G., Azevedo, P.N., Zanatta, Â., Plentz, I., Alcará, A.M. ... Da Costa, J.C. (2024). Influence of Zika virus on the cytotoxicity, cell adhesion, apoptosis and inflammatory markers of glioblastoma cells. Oncology Letters, 27, 176. https://doi.org/10.3892/ol.2024.14309
MLA
Marinowic, D. R., Zanirati, G. G., Azevedo, P. N., Zanatta, Â., Plentz, I., Alcará, A. M., Morrone, F. B., Scheffel, T. B., Cappellari, A. R., Roehe, P. M., Varela, A. M., Machado, D. C., Viola, F. S., Da Costa, J. C."Influence of Zika virus on the cytotoxicity, cell adhesion, apoptosis and inflammatory markers of glioblastoma cells". Oncology Letters 27.4 (2024): 176.
Chicago
Marinowic, D. R., Zanirati, G. G., Azevedo, P. N., Zanatta, Â., Plentz, I., Alcará, A. M., Morrone, F. B., Scheffel, T. B., Cappellari, A. R., Roehe, P. M., Varela, A. M., Machado, D. C., Viola, F. S., Da Costa, J. C."Influence of Zika virus on the cytotoxicity, cell adhesion, apoptosis and inflammatory markers of glioblastoma cells". Oncology Letters 27, no. 4 (2024): 176. https://doi.org/10.3892/ol.2024.14309