miR‑576‑3p/M‑phase phosphoprotein 8 axis regulates the malignant progression of hepatocellular carcinoma cells via the PI3K/Akt signaling pathway

Zhang,Nannan; Chi,Mengyi; Pan,Weili; Zhang,Congying; Wang,Yali; Gao,Xiaoyan; Bai,Chunying; Liu,Xianjun

doi:10.3892/ol.2024.14460

miR‑576‑3p/M‑phase phosphoprotein 8 axis regulates the malignant progression of hepatocellular carcinoma cells via the PI3K/Akt signaling pathway

Authors:
- Nannan Zhang
- Mengyi Chi
- Weili Pan
- Congying Zhang
- Yali Wang
- Xiaoyan Gao
- Chunying Bai
- Xianjun Liu
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Affiliations: Key Laboratory of Mechanism and Evaluation of Traditional Chinese & Mongolian Medicine, School of Basic Medicine, Chifeng University, Chifeng, Inner Mongolia Autonomous Region 024000, P.R. China, Department of Cardiovascular Medicine, The Second Hospital of Chifeng, Chifeng, Inner Mongolia Autonomous Region 024000, P.R. China, Key Laboratory of Research on Human Genetic Diseases at Universities of Inner Mongolia Autonomous Region, School of Basic Medicine, Chifeng University, Chifeng, Inner Mongolia Autonomous Region 024000, P.R. China, Department of Bioengineering, College of Biology and Food Engineering, Jilin Engineering Normal University, Changchun, Jilin 130000, P.R. China
Published online on: May 16, 2024 https://doi.org/10.3892/ol.2024.14460
Article Number: 327
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatocellular carcinoma (HCC) is a fatal digestive system cancer with unclear pathogenesis. M‑phase phosphoprotein 8 (MPP8) has been shown to play a vital role in several cancer types, such as non‑small cell lung cancer, gastric cancer and melanoma; however, there have been no studies into its role in HCC. The present study aimed to evaluate the role of MPP8 in regulating malignant phenotypes of liver cancer cells, and to further investigate the underlying mechanism. Bioinformatics analysis was performed to analyze related data from a public database, and to predict the potential microRNAs (miRNAs) that might target MPP8 mRNA; reverse transcription-quantitative PCR was used to measure the levels of mRNA and miRNA; western blotting was employed to detect protein levels; Cell Counting Kit‑8 (CCK‑8) and plate colony formation assays, wound healing assay and Transwell invasion assay were performed to evaluate the ability of cell proliferation, migration and invasion, respectively; dual‑luciferase reporter gene assay was performed to identify the target association. The results showed that MPP8 was a risk factor for the survival of patients with HCC, and was up‑regulated in HCC tissue samples and cell lines; MPP8 knockdown inhibited the proliferation, migration and invasion of liver cancer cells; MPP8 knockdown suppressed the PI3K/Akt pathway, and activation of this pathway reversed the inhibited liver cancer cell phenotypes by down‑regulating MPP8; miR‑576‑3p, which was low in liver cancer cells, negatively regulated MPP8 expression by directly targeting its mRNA; up‑regulating MPP8 expression reversed the inhibited signaling pathway and malignant phenotypes of liver cancer cells by miR‑576‑3p overexpression. In conclusion, the miR‑576‑3p/MPP8 axis regulates the proliferation, migration, and invasion of liver cancer cells through the PI3K/Akt signaling pathway. These findings lead novel insights into HCC progression, and propose MPP8 as a potential therapeutic target for HCC.

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