Open Access

Culture supernatant of Toxoplasma gondii tachyzoites inhibits the proliferation and metastasis of bladder cancer cells

  • Authors:
    • Shutong Du
    • Yiwei Yang
    • Tianfei Wu
    • Li Zhang
    • Hailong Li
    • Yan Lv
  • View Affiliations

  • Published online on: October 17, 2024     https://doi.org/10.3892/ol.2024.14757
  • Article Number: 12
  • Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Toxoplasma gondii, an opportunistic protozoan, can be cultivated from numerous human cell lines. Bladder cancer is the most common type of tumor in the urinary system and has a high incidence and mortality rate. Protozoan parasites have recently gained popularity as a research topic for cancer therapy. The present study aimed to evaluate the effect of T. gondii tachyzoite culture supernatants on bladder cancer cells. Bladder cancer 5637 cells were infected with two different genotypes of T. gondii tachyzoite culture supernatants, RH and ToxoDB#9. The effects of T. gondii tachyzoite culture supernatant on cell proliferation were detected using a Cell Counting Kit‑8 assay. The effects on cell migration and invasion were evaluated using wound healing and Transwell assays. The effect on the apoptosis rate of the cells was assessed using a TUNEL assay, while the protein levels of Bax and Bcl‑2 were analyzed by western blotting. The results indicated that the culture supernatant of T. gondii tachyzoites altered the morphology of bladder cancer 5637 cells, inhibited cell proliferation, reduced cell migration and invasion and promoted apoptosis. The ToxoDB#9‑type experimental group showed higher efficacy than the RH‑type experimental group in inhibiting bladder cancer cell proliferation, reducing cell migration and invasion and promoting apoptosis. These results indicated that the culture supernatant of T. gondii tachyzoites could be a promising therapeutic agent for the treatment of bladder cancer.
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January-2025
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Spandidos Publications style
Du S, Yang Y, Wu T, Zhang L, Li H and Lv Y: Culture supernatant of <em>Toxoplasma gondii</em> tachyzoites inhibits the proliferation and metastasis of bladder cancer cells. Oncol Lett 29: 12, 2025.
APA
Du, S., Yang, Y., Wu, T., Zhang, L., Li, H., & Lv, Y. (2025). Culture supernatant of <em>Toxoplasma gondii</em> tachyzoites inhibits the proliferation and metastasis of bladder cancer cells. Oncology Letters, 29, 12. https://doi.org/10.3892/ol.2024.14757
MLA
Du, S., Yang, Y., Wu, T., Zhang, L., Li, H., Lv, Y."Culture supernatant of <em>Toxoplasma gondii</em> tachyzoites inhibits the proliferation and metastasis of bladder cancer cells". Oncology Letters 29.1 (2025): 12.
Chicago
Du, S., Yang, Y., Wu, T., Zhang, L., Li, H., Lv, Y."Culture supernatant of <em>Toxoplasma gondii</em> tachyzoites inhibits the proliferation and metastasis of bladder cancer cells". Oncology Letters 29, no. 1 (2025): 12. https://doi.org/10.3892/ol.2024.14757