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Simultaneous detection of expression and gene mutations of HER2/neu in Chinese patients with gastric cancer

  • Authors:
    • Xiuli Zhang
    • Jianhui Qu
    • Gang Sun
    • Jing Yang
    • Yunsheng Yang

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  • Published online on: May 1, 2010     https://doi.org/10.3892/ol_00000099
  • Pages: 559-563
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Abstract

HER2/neu is one of the few identified oncogenes in tumorigenesis. Attention has been focused on the potential effect of HER2/neu mutations in the tyrosine kinase domain on carcinoma-targeted therapy. However, data concerning HER2/neu mutations in Chinese patients with gastric cancer (GC) are limited. This study aimed to detect the expression and somatic mutations of HER2/neu in Chinese patients with GC. Immunohistochemical staining for HER2/neu was performed on 72 formalin-fixed, paraffin-embedded specimens of GC (40 intestinal and 32 diffuse type). The correlation between the overexpression of HER2/neu and clinicopathological parameters was statistically analyzed. Somatic mutations in the tyrosine kinase domain of HER2/neu in the 72 patients were detected by direct sequencing. In the GC group, overexpression of HER2/neu was detected in 13 of the 72 GC patients and in 4 of the 72 adjacent tissues in the non-tumorous group (18.1 vs. 5.6%, P<0.05). Furthermore, the intestinal type of GC exhibited a higher rate of HER2/neu overexpression than the diffuse type (29.7 vs. 5.7%, P<0.05). The rate of HER2/neu overexpression in stage III-IV (TNM stage) GC cases was significantly higher than that in stage I-II (28.2 vs. 6.6%, P<0.05). HER2/neu overexpression correlated with a significantly less favorable patient survival (P=0.046). No somatic mutations in the tyrosine kinase domain of HER2/neu were detected in tumor tissues or the corresponding non-tumorous ones in the specimens obtained from the 72 Chinese GC patients. Results suggest that overexpression of HER2/neu is a frequent molecular event strongly associated with a poor patient prognosis, whereas the incidence of somatic mutations of the HER2/neu kinase domain is more likely a low-frequency event in Chinese GC patients.
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May-June 2010
Volume 1 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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  • Impact Factor: 2.5
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  • CiteScore: 5.7
  • CiteScore Rank: Q2: #139/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.574
  • SCImago Journal Rank (SJR): 0.644
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Spandidos Publications style
Zhang X, Qu J, Sun G, Yang J and Yang Y: Simultaneous detection of expression and gene mutations of HER2/neu in Chinese patients with gastric cancer . Oncol Lett 1: 559-563, 2010.
APA
Zhang, X., Qu, J., Sun, G., Yang, J., & Yang, Y. (2010). Simultaneous detection of expression and gene mutations of HER2/neu in Chinese patients with gastric cancer . Oncology Letters, 1, 559-563. https://doi.org/10.3892/ol_00000099
MLA
Zhang, X., Qu, J., Sun, G., Yang, J., Yang, Y."Simultaneous detection of expression and gene mutations of HER2/neu in Chinese patients with gastric cancer ". Oncology Letters 1.3 (2010): 559-563.
Chicago
Zhang, X., Qu, J., Sun, G., Yang, J., Yang, Y."Simultaneous detection of expression and gene mutations of HER2/neu in Chinese patients with gastric cancer ". Oncology Letters 1, no. 3 (2010): 559-563. https://doi.org/10.3892/ol_00000099