Effect of Fraxiparine, a type of low molecular weight heparin, on the invasion and metastasis of lung adenocarcinoma A549 cells

  • Authors:
    • Chuan-Jiang Yu
    • Su-Juan Ye
    • Zhi-Hua Feng
    • Wen-Jing Ou
    • Xi-Kun Zhou
    • Ling-Dong Li
    • Yong-Qiu Mao
    • Wen Zhu
    • Yu-Quan Wei
  • View Affiliations

  • Published online on: July 1, 2010     https://doi.org/10.3892/ol_00000132
  • Pages: 755-760
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Abstract

Lung cancer is one of the most highly malignant tumors, and a significant threat to human health. Lung cancer patients often exhibit tumor cell invasion and metastasis, which often render current treatments ineffective. Recently, the beneficial effects of low molecular weight heparin (LMWH) on cancer metastasis were reported in pre-clinical research studies. LMWH may be a potential drug for cancer therapy. However, the mechanism of LMWH on the invasion and metastasis of cancer has yet to be determined. This study investigated the effects of Fraxiparine on the proliferation, invasion and metastasis of the human lung adenocarcinoma A549 cell line. MTT assay and flow cytometry showed that Fraxiparine slightly inhibited the cell viability dose- and time-dependently, but did not arrest the A549 cells in the G1 phase nor induce early apoptosis. The transwell chamber assay showed that Fraxiparine significantly suppressed the invasion and migration of the A549 cells in vitro. Fraxiparine also markedly inhibited the adhesion of the A549 cells to Matrigel. The RT-PCR assay demonstrated that the reduction in invasion and metastasis may be related to the up-regulation of nm23-H1 and the down-regulation of the heparanase expression. Moreover, the RT-PCR assay and Western blot analysis demonstrated that down-regulation of the expression of integrin β1 and β3, as well as that of matrix metalloproteinase-2 and -9 may be responsible for the inhibition of the invasion and metastasis of A549 cells by Fraxiparine.
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July-August 2010
Volume 1 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yu C, Ye S, Feng Z, Ou W, Zhou X, Li L, Mao Y, Zhu W and Wei Y: Effect of Fraxiparine, a type of low molecular weight heparin, on the invasion and metastasis of lung adenocarcinoma A549 cells . Oncol Lett 1: 755-760, 2010.
APA
Yu, C., Ye, S., Feng, Z., Ou, W., Zhou, X., Li, L. ... Wei, Y. (2010). Effect of Fraxiparine, a type of low molecular weight heparin, on the invasion and metastasis of lung adenocarcinoma A549 cells . Oncology Letters, 1, 755-760. https://doi.org/10.3892/ol_00000132
MLA
Yu, C., Ye, S., Feng, Z., Ou, W., Zhou, X., Li, L., Mao, Y., Zhu, W., Wei, Y."Effect of Fraxiparine, a type of low molecular weight heparin, on the invasion and metastasis of lung adenocarcinoma A549 cells ". Oncology Letters 1.4 (2010): 755-760.
Chicago
Yu, C., Ye, S., Feng, Z., Ou, W., Zhou, X., Li, L., Mao, Y., Zhu, W., Wei, Y."Effect of Fraxiparine, a type of low molecular weight heparin, on the invasion and metastasis of lung adenocarcinoma A549 cells ". Oncology Letters 1, no. 4 (2010): 755-760. https://doi.org/10.3892/ol_00000132