Expression of a novel splicing variant deleting exons 4 and 6 of the progesterone receptor gene is a rare event in breast cancer

  • Authors:
    • Kumi Nagao
    • Norio Kohno
    • Kazuyuki Wakita
    • Kazumasa Hikiji
    • Shigeki Yamamoto
    • Hiroyuki Hirata
    • Hisashi Hisatomi
  • View Affiliations

  • Published online on: March 1, 2003     https://doi.org/10.3892/or.10.2.305
  • Pages: 305-308
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Abstract

We identified a novel alternatively spliced isoform of PR mRNA in breast cancer tissues. The deleted transcript was characterized by an out-of-frame deletion of 437 bp, corresponding to the complete loss of exons 4 and 6 (PR delta4+6 ASV). PR delta4+6 ASV will result in a partial defect in the region of the ligand-binding domain of hormone receptors, suggesting that the conserved residues are missing from the core of the protein. In the limited number of samples studied, a novel PR delta4+6 mRNA was detected in 1 of 45 (2.2%) non-cancerous tissues of patients with breast cancer, in 5 of 45 (11.1%) cancerous tissues of patients with breast cancer. Loss of both exons 4 and 6 will be induced by incomplete splicing and/or repair mechanism. Further studies are necessary to establish the biological significance of this alternative splicing. The expressions of ASVs that induced the mimic PR transcripts need to be considered when designing strategies for regulation analysis of the PR gene.

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March-April 2003
Volume 10 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Nagao K, Kohno N, Wakita K, Hikiji K, Yamamoto S, Hirata H and Hisatomi H: Expression of a novel splicing variant deleting exons 4 and 6 of the progesterone receptor gene is a rare event in breast cancer. Oncol Rep 10: 305-308, 2003.
APA
Nagao, K., Kohno, N., Wakita, K., Hikiji, K., Yamamoto, S., Hirata, H., & Hisatomi, H. (2003). Expression of a novel splicing variant deleting exons 4 and 6 of the progesterone receptor gene is a rare event in breast cancer. Oncology Reports, 10, 305-308. https://doi.org/10.3892/or.10.2.305
MLA
Nagao, K., Kohno, N., Wakita, K., Hikiji, K., Yamamoto, S., Hirata, H., Hisatomi, H."Expression of a novel splicing variant deleting exons 4 and 6 of the progesterone receptor gene is a rare event in breast cancer". Oncology Reports 10.2 (2003): 305-308.
Chicago
Nagao, K., Kohno, N., Wakita, K., Hikiji, K., Yamamoto, S., Hirata, H., Hisatomi, H."Expression of a novel splicing variant deleting exons 4 and 6 of the progesterone receptor gene is a rare event in breast cancer". Oncology Reports 10, no. 2 (2003): 305-308. https://doi.org/10.3892/or.10.2.305