Hot water soluble sesquiterpenes [anhydroperoxy-costunolide and 3-oxoeudesma-1,4(15),11(13)triene-12,6α-olide] isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia cells

  • Authors:
    • Takashi Komiya
    • Yayoi Yamada
    • Hiroyuki Moteki
    • Hirotaka Katsuzaki
    • Kunio Imai
    • Hiroshige Hibasami
  • View Affiliations

  • Published online on: January 1, 2004     https://doi.org/10.3892/or.11.1.85
  • Pages: 85-88
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Abstract

Hot water soluble (HWS)-sesquiterpenes [anhydroperoxycostunolide and 3-oxo-eudesma-1,4(15),11(13)triene-12,6α-olide] were purified from Laurel (Laurus nobilis L.) and identified by Mass, and 1H- and 13C-NMR. These HWS-sesquiterpenes displayed strong growth inhibitory effect against human promyelotic leukemia HL-60 cells. Apoptotic morphological changes of the nucleus, including chromatin condensation were induced in the HL-60 cells treated with the sesquiterpenes. Flow cytometric analysis showed that the hypodiploid nuclei of HL-60 cells were increased to 10.5, 46.5, and 91.3% after a 3 day-treatment with 2.5, 5 and 10 µM anhydroperoxycostunolide, respectively. And the same analysis showed that the hypodiploid nuclei of HL-60 cells were increased to 9.8, 39.2 and 89.6% after a 3 day-treatment with 5, 10 and 20 µM 3-oxo-eudesma-1,4(15),11(13)triene-12,6α-olide, respectively. These findings suggest that growth inhibition by anhydroperoxycostunolide and 3-oxo-eudesma-1,4(15),11(13)triene-12,6α-olide of HL-60 cells results from the induction of chromatin condensation in the HL-60 cells. On the other hand, fragmentations by these compounds of DNA to oligonucleosomal-sized fragments were not observed in the HL-60 cells.

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January 2004
Volume 11 Issue 1

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Spandidos Publications style
Komiya T, Yamada Y, Moteki H, Katsuzaki H, Imai K and Hibasami H: Hot water soluble sesquiterpenes [anhydroperoxy-costunolide and 3-oxoeudesma-1,4(15),11(13)triene-12,6α-olide] isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia cells. Oncol Rep 11: 85-88, 2004.
APA
Komiya, T., Yamada, Y., Moteki, H., Katsuzaki, H., Imai, K., & Hibasami, H. (2004). Hot water soluble sesquiterpenes [anhydroperoxy-costunolide and 3-oxoeudesma-1,4(15),11(13)triene-12,6α-olide] isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia cells. Oncology Reports, 11, 85-88. https://doi.org/10.3892/or.11.1.85
MLA
Komiya, T., Yamada, Y., Moteki, H., Katsuzaki, H., Imai, K., Hibasami, H."Hot water soluble sesquiterpenes [anhydroperoxy-costunolide and 3-oxoeudesma-1,4(15),11(13)triene-12,6α-olide] isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia cells". Oncology Reports 11.1 (2004): 85-88.
Chicago
Komiya, T., Yamada, Y., Moteki, H., Katsuzaki, H., Imai, K., Hibasami, H."Hot water soluble sesquiterpenes [anhydroperoxy-costunolide and 3-oxoeudesma-1,4(15),11(13)triene-12,6α-olide] isolated from laurel (Laurus nobilis L.) induce cell death and morphological change indicative of apoptotic chromatin condensation in leukemia cells". Oncology Reports 11, no. 1 (2004): 85-88. https://doi.org/10.3892/or.11.1.85