Role of complex cadherins in cell-cell adhesion evaluated by spheroid formation in renal cell carcinoma cell lines

  • Authors:
    • Toru Shimazui
    • Jack A. Schalken
    • Koji Kawai
    • Risa Kawamoto
    • Adrie Van Bockhoven
    • Egbert Oosterwijk
    • Hideyuki Akaza
  • View Affiliations

  • Published online on: February 1, 2004     https://doi.org/10.3892/or.11.2.357
  • Pages: 357-360
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Abstract

We have previously shown that renal cell carcinoma (RCC) cell lines expressed a complex set of cadherins, e.g. E-cadherin, N-cadherin and cadherin-6. It is also reported that E-cadherin and cadherin-6 have a predictive value for estimating a patient's prognosis in RCC. However, E-cadherin is infrequently expressed in RCC as compared with N-cadherin and cadherin-6. In the present study, therefore, we performed a functional analysis of these cadherins as a cell adhesion molecule using spheroid culturing and spheroid-blocking assay. In E-cadherin expressers, compact spheroid formation was observed, and it was inhibited by anti-E-cadherin antibody. In contrast, in E-cadherin-absent lines, cadherin-6 apparently played a role to form relatively loose spheroids and this spheroid formation was inhibited by anti-cadherin-6 antibody. In both spheroids, the anti-N-cadherin antibody could not inhibit their formation, suggesting that N-cadherin was not an essential molecule for spheroid formation in the cell lines expressing complex cadherins. The anti-N-cadherin antibody inhibited spheroid formation only in the cell line that expressed N-cadherin alone. Using western blot analysis, immunohistochemistry and immunoprecipitation, these cadherins were linked to catenins to make a functional adhesion molecule. In conclusion, E-cadherin and cadherin-6 are shown to act in cell-cell adhesion whereas N-cadherin might play a somewhat different role from cell-cell adhesion in RCC cell lines.

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February 2004
Volume 11 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Shimazui T, Schalken JA, Kawai K, Kawamoto R, Van Bockhoven A, Oosterwijk E and Akaza H: Role of complex cadherins in cell-cell adhesion evaluated by spheroid formation in renal cell carcinoma cell lines. Oncol Rep 11: 357-360, 2004.
APA
Shimazui, T., Schalken, J.A., Kawai, K., Kawamoto, R., Van Bockhoven, A., Oosterwijk, E., & Akaza, H. (2004). Role of complex cadherins in cell-cell adhesion evaluated by spheroid formation in renal cell carcinoma cell lines. Oncology Reports, 11, 357-360. https://doi.org/10.3892/or.11.2.357
MLA
Shimazui, T., Schalken, J. A., Kawai, K., Kawamoto, R., Van Bockhoven, A., Oosterwijk, E., Akaza, H."Role of complex cadherins in cell-cell adhesion evaluated by spheroid formation in renal cell carcinoma cell lines". Oncology Reports 11.2 (2004): 357-360.
Chicago
Shimazui, T., Schalken, J. A., Kawai, K., Kawamoto, R., Van Bockhoven, A., Oosterwijk, E., Akaza, H."Role of complex cadherins in cell-cell adhesion evaluated by spheroid formation in renal cell carcinoma cell lines". Oncology Reports 11, no. 2 (2004): 357-360. https://doi.org/10.3892/or.11.2.357