Frequent CYP1A1 expression in gastric cancers and their related lesions

  • Authors:
    • Kai-Li Zhang
    • Jing-Xin Ma
    • Xiao-Yan Chen
    • Yuan Sun
    • Qing-You Kong
    • Jia Liu
    • Hong Li
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/or.12.6.1335
  • Pages: 1335-1340
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Abstract

CYP1A1 expression in various tissues of 43 gastric cancer cases was analyzed with frozen tissue array-based immunohistochemical staining (IHC), RT-PCR, Western blot analysis and EROD (7-ethoxyresourfin-O-deethylase) assay. CYP1A1 was detected immunohistochemically in 86% (37/43) of gastric cancers, in both 57% (4/7) of atrophic gastritis and intestinal metaplasia and in 7.7% (1/13) of non-cancerous mucosa. RT-PCR and Western blot analysis revealed coincident results with that of IHC and ruled out possible concurrent CYP1A2 expression. EROD assay showed increased CYP1A1/1B1 activity in either cancer or premalignant tissues but not in non-cancerous ones. The frequencies of CYP1A1 expression are significantly different between non-cancerous and premalignant (P<0.025) or cancer groups (P<0.005), suggesting that CYP1A1 is expressed at relatively early stage of gastrocarcinogenesis and exerts its effects throughout the stepwise oncogenic processes.

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December 2004
Volume 12 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang K, Ma J, Chen X, Sun Y, Kong Q, Liu J and Li H: Frequent CYP1A1 expression in gastric cancers and their related lesions. Oncol Rep 12: 1335-1340, 2004.
APA
Zhang, K., Ma, J., Chen, X., Sun, Y., Kong, Q., Liu, J., & Li, H. (2004). Frequent CYP1A1 expression in gastric cancers and their related lesions. Oncology Reports, 12, 1335-1340. https://doi.org/10.3892/or.12.6.1335
MLA
Zhang, K., Ma, J., Chen, X., Sun, Y., Kong, Q., Liu, J., Li, H."Frequent CYP1A1 expression in gastric cancers and their related lesions". Oncology Reports 12.6 (2004): 1335-1340.
Chicago
Zhang, K., Ma, J., Chen, X., Sun, Y., Kong, Q., Liu, J., Li, H."Frequent CYP1A1 expression in gastric cancers and their related lesions". Oncology Reports 12, no. 6 (2004): 1335-1340. https://doi.org/10.3892/or.12.6.1335