Glycosylation modulates TRAIL-R1/death receptor 4 protein: different regulations of two pro-apoptotic receptors for TRAIL by tunicamycin

  • Authors:
    • Tatsushi Yoshida
    • Takumi Shiraishi
    • Mano Horinaka
    • Miki Wakada
    • Toshiyuki Sakai
  • View Affiliations

  • Published online on: November 1, 2007     https://doi.org/10.3892/or.18.5.1239
  • Pages: 1239-1242
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Abstract

Death receptor 4 (DR4) is a receptor of the antitumor death ligand, TNF-related apoptosis-inducing ligand (TRAIL), and is considered a promising molecular target for cancer therapy. Here, we show a novel regulation of DR4 protein. Tunicamycin treatment, which is an inducer of endoplasmic reticulum (ER)-stress, generated a lower molecular-weight pattern of DR4, but not DR5 protein in prostate cancer DU145 and PC3 cells. Thus, we termed the small form of DR4 protein, DR4-Small (DR4-S) and the large form, DR4-Large (DR4-L). Using DR4 siRNA, we confirmed that DR4-S also stands for DR4 protein. Other ER-stress inducers, brefeldin A and thapsigargin did not generate DR4-S. On the other hand, these ER-stress inducers increased DR5 protein. Tunicamycin induces ER-stress following the inhibition of N-linked glycosylation. Thus, we examined DR4 protein in cell lysates treated with glycosydase. Glycosydase treatments generated DR4-S protein, similar to tunicamycin. These results indicate that tunicamycin regulates DR4 protein size via inhibition of glycosylation.

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November 2007
Volume 18 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yoshida T, Shiraishi T, Horinaka M, Wakada M and Sakai T: Glycosylation modulates TRAIL-R1/death receptor 4 protein: different regulations of two pro-apoptotic receptors for TRAIL by tunicamycin. Oncol Rep 18: 1239-1242, 2007.
APA
Yoshida, T., Shiraishi, T., Horinaka, M., Wakada, M., & Sakai, T. (2007). Glycosylation modulates TRAIL-R1/death receptor 4 protein: different regulations of two pro-apoptotic receptors for TRAIL by tunicamycin. Oncology Reports, 18, 1239-1242. https://doi.org/10.3892/or.18.5.1239
MLA
Yoshida, T., Shiraishi, T., Horinaka, M., Wakada, M., Sakai, T."Glycosylation modulates TRAIL-R1/death receptor 4 protein: different regulations of two pro-apoptotic receptors for TRAIL by tunicamycin". Oncology Reports 18.5 (2007): 1239-1242.
Chicago
Yoshida, T., Shiraishi, T., Horinaka, M., Wakada, M., Sakai, T."Glycosylation modulates TRAIL-R1/death receptor 4 protein: different regulations of two pro-apoptotic receptors for TRAIL by tunicamycin". Oncology Reports 18, no. 5 (2007): 1239-1242. https://doi.org/10.3892/or.18.5.1239