pseudo-G-Rh2 induces mitochondrial-mediated apoptosis in SGC-7901 human gastric cancer cells

  • Authors:
    • Xiangru Qu
    • Shaochun Qu
    • Xiaofeng Yu
    • Huali Xu
    • Yanping Chen
    • Xingyuan Ma
    • Dayuan Sui
  • View Affiliations

  • Published online on: August 31, 2011     https://doi.org/10.3892/or.2011.1442
  • Pages: 1441-1446
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Abstract

This study was designed to investigate the effect of pseudo-G-Rh2, a novel metabolite of ginsenoside Rh2, on the apoptosis of SGC-7901 human gastric cancer cells. Pseudo-G-Rh2 demonstrated antitumor activity and significantly inhibited the proliferation of SGC-7901 cells in a concentration-dependent manner. After treatment with pseudo-G-Rh2, SGC-7901 cells showed typical apoptotic morphological features, such as chromatin condensation and DNA fragmentation. Pseudo-G-Rh2 could induce mitochondrial membrane potential loss, which led to the release of cytochrome c (Cyt c), Smac/Diablo and apoptosis-inducing factor (AIF) to the cell cytoplasm. Furthermore, pseudo-G-Rh2 exposure not only decreased the expression of the Bcl-2 protein but also increased the expression of the Bax protein and the activities of caspase-9 and caspase-3 in SGC-7901 cells. These results demonstrated that pseudo-G-Rh2 inhibited the proliferation of SGC-7901 cells by initiating apoptosis. Pseudo-G-Rh2-induced apoptosis was associated with a drop in the mitochondrial transmembrane potential, down-regulation of Bcl-2, up-regulation of Bax and activation of caspase-9 and caspase-3.

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December 2011
Volume 26 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Qu X, Qu S, Yu X, Xu H, Chen Y, Ma X and Sui D: pseudo-G-Rh2 induces mitochondrial-mediated apoptosis in SGC-7901 human gastric cancer cells. Oncol Rep 26: 1441-1446, 2011.
APA
Qu, X., Qu, S., Yu, X., Xu, H., Chen, Y., Ma, X., & Sui, D. (2011). pseudo-G-Rh2 induces mitochondrial-mediated apoptosis in SGC-7901 human gastric cancer cells. Oncology Reports, 26, 1441-1446. https://doi.org/10.3892/or.2011.1442
MLA
Qu, X., Qu, S., Yu, X., Xu, H., Chen, Y., Ma, X., Sui, D."pseudo-G-Rh2 induces mitochondrial-mediated apoptosis in SGC-7901 human gastric cancer cells". Oncology Reports 26.6 (2011): 1441-1446.
Chicago
Qu, X., Qu, S., Yu, X., Xu, H., Chen, Y., Ma, X., Sui, D."pseudo-G-Rh2 induces mitochondrial-mediated apoptosis in SGC-7901 human gastric cancer cells". Oncology Reports 26, no. 6 (2011): 1441-1446. https://doi.org/10.3892/or.2011.1442