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miRNA-195 sensitizes human hepatocellular carcinoma cells to 5-FU by targeting BCL-w

  • Authors:
    • Xiaoyan Yang
    • Jie Yin
    • Jia Yu
    • Qiong Xiang
    • Yunmei Liu
    • Shensong Tang
    • Duanfang Liao
    • Bingyang Zhu
    • Xuyu Zu
    • Huifang Tang
    • Xiaoyong Lei

  • View Affiliations

  • Published online on: September 22, 2011     https://doi.org/10.3892/or.2011.1472
  • Pages: 250-257
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Abstract

The role of microRNA-195 in developing acquired drug resistance in hepatocellular carcinoma cells was investigated. Expression profiling of miRNAs revealed a limited set of miRNAs with altered expression in drug resistant hepatocellular carcinoma cell line BEL-7402/5-FU compared to its parental BEL-7402 cell line. Real-time PCR confirmed down-regulation of miRNA-195 in BEL-7402/5-FU cells. Overexpression of miRNA-195 sensitized BEL-7402/5-FU cells to anticancer drugs. Consistent with these findings, miR-195 antisense oligonucleotide induced drug resistance in BEL-7402/5-FU cells. Also, the basal levels of the anti-apoptotic protein Bcl-w were high in BEL-7402/5-FU cells and miR-195 overexpression repressed Bcl-w protein level and inhibited the luciferase activity of a Bcl-w 3' untranslated region-based reporter construct in both BEL-7402/5-FU and BEL-7402 cells. These results indicate that miR-195 could improve the drug sensitivity at least in part by targeting Bcl-w to increase cell apoptosis in hepatocellular carcinoma cells.

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January 2012
Volume 27 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • CiteScore Rank: Q1: #74/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
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Spandidos Publications style
Yang X, Yin J, Yu J, Xiang Q, Liu Y, Tang S, Liao D, Zhu B, Zu X, Tang H, Tang H, et al: miRNA-195 sensitizes human hepatocellular carcinoma cells to 5-FU by targeting BCL-w. Oncol Rep 27: 250-257, 2012.
APA
Yang, X., Yin, J., Yu, J., Xiang, Q., Liu, Y., Tang, S. ... Lei, X. (2012). miRNA-195 sensitizes human hepatocellular carcinoma cells to 5-FU by targeting BCL-w. Oncology Reports, 27, 250-257. https://doi.org/10.3892/or.2011.1472
MLA
Yang, X., Yin, J., Yu, J., Xiang, Q., Liu, Y., Tang, S., Liao, D., Zhu, B., Zu, X., Tang, H., Lei, X."miRNA-195 sensitizes human hepatocellular carcinoma cells to 5-FU by targeting BCL-w". Oncology Reports 27.1 (2012): 250-257.
Chicago
Yang, X., Yin, J., Yu, J., Xiang, Q., Liu, Y., Tang, S., Liao, D., Zhu, B., Zu, X., Tang, H., Lei, X."miRNA-195 sensitizes human hepatocellular carcinoma cells to 5-FU by targeting BCL-w". Oncology Reports 27, no. 1 (2012): 250-257. https://doi.org/10.3892/or.2011.1472