VI-16, a newly synthesized flavonoid, induces apoptosis through the mitochondrial pathway in human hepatoma cells

  • Authors:
    • Na Lu
    • Libin Wei
    • Dandan Gong
    • Yuan Gao
    • Qinsheng Dai
    • Zhiyu Li
    • Qinglong Guo
  • View Affiliations

  • Published online on: December 14, 2011     https://doi.org/10.3892/or.2011.1594
  • Pages: 873-879
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Abstract

VI-16, a newly synthesized flavonoid, has a hydroxy substitution at C5 position, a methoxyl substitution at C5 position, and a piperazine substitution at C7 position. Here, we firstly investigated the potential antitumor effect of VI-16 in HepG2 human hepatocarcinoma cells. The MTT assay showed that VI-16 inhibited HepG2 cell growth in a concentration- and time-dependent manner. To further investigate whether apoptosis induction contributed to the antitumor effects of VI-16, DAPI staining and Annexin-V/PI double staining were performed in our tests. The data showed that VI-16 could induce apoptotic cell death in HepG2 cells. Moreover, mechanistic studies revealed that VI-16-induced apoptosis was a caspase-dependent process by decreasing the expression of pro-caspase-3. The changes in the expression of caspase-8, capsase-9, Bax and bcl-2 after VI-16 treatment suggested that the mitochondrial pathway was involved in the apoptosis induced by VI-16. Furthermore, VI-16 could significantly increase the loss of mitochondrial membrane potential and the expression of p53. Taken together, these results demonstrated that apoptosis induced by VI-16 might be one of the mechanisms by which VI-16 acts as a preventive antitumor drug against human hepatoma.

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March 2012
Volume 27 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lu N, Wei L, Gong D, Gao Y, Dai Q, Li Z and Guo Q: VI-16, a newly synthesized flavonoid, induces apoptosis through the mitochondrial pathway in human hepatoma cells. Oncol Rep 27: 873-879, 2012.
APA
Lu, N., Wei, L., Gong, D., Gao, Y., Dai, Q., Li, Z., & Guo, Q. (2012). VI-16, a newly synthesized flavonoid, induces apoptosis through the mitochondrial pathway in human hepatoma cells. Oncology Reports, 27, 873-879. https://doi.org/10.3892/or.2011.1594
MLA
Lu, N., Wei, L., Gong, D., Gao, Y., Dai, Q., Li, Z., Guo, Q."VI-16, a newly synthesized flavonoid, induces apoptosis through the mitochondrial pathway in human hepatoma cells". Oncology Reports 27.3 (2012): 873-879.
Chicago
Lu, N., Wei, L., Gong, D., Gao, Y., Dai, Q., Li, Z., Guo, Q."VI-16, a newly synthesized flavonoid, induces apoptosis through the mitochondrial pathway in human hepatoma cells". Oncology Reports 27, no. 3 (2012): 873-879. https://doi.org/10.3892/or.2011.1594