AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice

He,Sha-Sha; Shi,Hua-Shan; Yin,Tao; Li,Yong-Xia; Luo,Shun-Tao; Wu,Qin-Jie; Lu,Lian; Wei,Yu-Quan; Yang,Li

doi:10.3892/or.2012.1621

AAV-mediated gene transfer of human pigment epithelium-derived factor inhibits lewis lung carcinoma growth in mice

Authors:
- Sha-Sha He
- Hua-Shan Shi
- Tao Yin
- Yong-Xia Li
- Shun-Tao Luo
- Qin-Jie Wu
- Lian Lu
- Yu-Quan Wei
- Li Yang
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Affiliations: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, P.R. China, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Keyuan Road 4, Chengdu, Sichuan 610041, P.R. China
Published online on: January 4, 2012 https://doi.org/10.3892/or.2012.1621
Pages: 1142-1148

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Abstract

Pigment epithelium-derived factor (PEDF) is the most potent inhibitor of angiogenesis in the mammalian eye, and mechanisms through which PEDF exerts its antitumour activity have recently been defined. The aim of our research was to evaluate the ability of adeno-associated virus (AAV) vector-mediated transfer of human PEDF to inhibit lewis lung carcinoma (LCC) cell growth. Intratumoural injection of AAV-PEDF caused significant reduction of the tumour volume and prolonged the survival time of mice bearing LLC cells, which were associated with decreased microvessel density and increased apoptosis in the tumours. AAV vectors represent a very promising tool for cancer gene therapy. No noticeable toxicity concerning AAV was detected as inferred from monitoring changes in animal body weight as well as basic organ structure and histological morphology, and by analyzing mouse liver and kidney function. Our findings indicate that AAV-mediated PEDF gene expression may offer an active approach to inhibit LLC growth and that treatment with AAV-PEDF may provide a promising therapeutic strategy in lung cancer treatment.

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