Open Access

Silencing of the hTERT gene through RNA interference induces apoptosis via bax/bcl-2 in human glioma cells

  • Authors:
    • Tuo Wang
    • Yan Xue
    • Maode Wang
    • Qiang Sun
  • View Affiliations

  • Published online on: August 6, 2012     https://doi.org/10.3892/or.2012.1952
  • Pages: 1153-1158
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Glioma cells are characterized by their invasiveness and resistance to conventional therapeutics. The downregulation of human telomerase reverse transcriptase (hTERT) can lead to decreased cell proliferation and/or the induction of apoptotic cell death in cancer cells but has rarely been reported in glioma cells. Here, we assessed the effect of the silencing of the hTERT gene on cell apoptosis and its possible molecular mechanism in T98G glioma cells. We found that the silencing of the hTERT gene in T98G cells significantly decreased cell proliferation and telomerase activity, increased the number of cells in G1 phase and decreased the number of cells in S phase, and induced apoptosis via decreasing the protein level of bcl-2 and c-myc and increasing the protein levels of bax and p53.
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October 2012
Volume 28 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Wang T, Xue Y, Wang M and Sun Q: Silencing of the hTERT gene through RNA interference induces apoptosis via bax/bcl-2 in human glioma cells. Oncol Rep 28: 1153-1158, 2012.
APA
Wang, T., Xue, Y., Wang, M., & Sun, Q. (2012). Silencing of the hTERT gene through RNA interference induces apoptosis via bax/bcl-2 in human glioma cells. Oncology Reports, 28, 1153-1158. https://doi.org/10.3892/or.2012.1952
MLA
Wang, T., Xue, Y., Wang, M., Sun, Q."Silencing of the hTERT gene through RNA interference induces apoptosis via bax/bcl-2 in human glioma cells". Oncology Reports 28.4 (2012): 1153-1158.
Chicago
Wang, T., Xue, Y., Wang, M., Sun, Q."Silencing of the hTERT gene through RNA interference induces apoptosis via bax/bcl-2 in human glioma cells". Oncology Reports 28, no. 4 (2012): 1153-1158. https://doi.org/10.3892/or.2012.1952