β-elemene induces glioma cell apoptosis by downregulating survivin and its interaction with hepatitis B X-interacting protein

Zhang,Hang; Xu,Feng; Xie,Tian; Jin,Hongchuan; Shi,Liyun

doi:10.3892/or.2012.2022

β-elemene induces glioma cell apoptosis by downregulating survivin and its interaction with hepatitis B X-interacting protein

Authors:
- Hang Zhang
- Feng Xu
- Tian Xie
- Hongchuan Jin
- Liyun Shi
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Affiliations: Department of Basic Medical Science, Hangzhou Normal University, Hangzhou, Zhejiang, P.R. China, Department of Respiratory Medicine, Second Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, P.R. Chin, Research Center for Biomedicine and Health, Hangzhou Normal University, Hangzhou, Zhejiang, P.R. China, Laboratory of Cancer Epigenetics, Key Laboratory of Biotherapy in Zhejiang Province, Biomedical Research Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, P.R. China
Published online on: September 11, 2012 https://doi.org/10.3892/or.2012.2022
Pages: 2083-2090

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Abstract

β-elemene, extracted from the ginger plant, possesses antitumor activity against a broad range of cancers clinically. However, the mechanism underlying β-elemene-induced cytotoxicity remains incompletely understood. Here, we show that β-elemene promoted apoptotic cell death in human glioma cells, downregulated survivin gene expression, and induced caspase-9, -3 and -7 activities. Induction of apoptosis was associated with inhibition of survivin gene expression, and restoration of survivin levels remarkably attenuated β-elemene-induced glioma cell death. Moreover, we found that the interaction between surviving and HBXIP, a critical regulator of caspase-9 activity, was impaired by β-elemene treatment. The results, therefore, reveal a caspase-mediated apoptotic pathway induced by β-elemene in human glioma cells, which is associated with downregulation of survivin itself and the interaction between survivin and HBXP.

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