Downregulation of Ezh2 expression by RNA interference induces cell cycle arrest in the G0/G1 phase and apoptosis in U87 human glioma cells

  • Authors:
    • Ruijian Zhang
    • Ruijun Wang
    • Hong Chang
    • Fei Wu
    • Chuntao Liu
    • Dongfeng Deng
    • Wenhai Fan
  • View Affiliations

  • Published online on: September 17, 2012     https://doi.org/10.3892/or.2012.2033
  • Pages: 2278-2284
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Abstract

The Ezh2 gene is an important member of the polycomb-group (PcG) family. As a newly identified oncogene, the expression of Ezh2 has been shown to be significantly increased in prostate cancer, breast cancer, renal cell carcinoma and hepatic cancer; however, a role for Ezh2 in the occurrence of glioma has not yet been reported. In this study, we found that the Ezh2 gene is highly expressed in U87 human glioma cells. Using RNA interference, we demonstrated that the downregulation of Ezh2 expression in U87 human glioma cells resulted in apoptosis and a cell cycle arrest in the G0/G1 phase. In addition, we found that silencing of the Ezh2 gene altered the mitochondrial membrane potential and promoted the release of cytochrome c from the mitochondria. Furthermore, the reduced expression of Ezh2 altered the Bax and Bcl-2 protein levels and led to the activation of caspase 9 and 3. These results indicate that the apoptosis induced in U87 human glioma cells by the silencing of the Ezh2 gene is related to the mitochondrial pathway.
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December 2012
Volume 28 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang R, Wang R, Chang H, Wu F, Liu C, Deng D and Fan W: Downregulation of Ezh2 expression by RNA interference induces cell cycle arrest in the G0/G1 phase and apoptosis in U87 human glioma cells. Oncol Rep 28: 2278-2284, 2012.
APA
Zhang, R., Wang, R., Chang, H., Wu, F., Liu, C., Deng, D., & Fan, W. (2012). Downregulation of Ezh2 expression by RNA interference induces cell cycle arrest in the G0/G1 phase and apoptosis in U87 human glioma cells. Oncology Reports, 28, 2278-2284. https://doi.org/10.3892/or.2012.2033
MLA
Zhang, R., Wang, R., Chang, H., Wu, F., Liu, C., Deng, D., Fan, W."Downregulation of Ezh2 expression by RNA interference induces cell cycle arrest in the G0/G1 phase and apoptosis in U87 human glioma cells". Oncology Reports 28.6 (2012): 2278-2284.
Chicago
Zhang, R., Wang, R., Chang, H., Wu, F., Liu, C., Deng, D., Fan, W."Downregulation of Ezh2 expression by RNA interference induces cell cycle arrest in the G0/G1 phase and apoptosis in U87 human glioma cells". Oncology Reports 28, no. 6 (2012): 2278-2284. https://doi.org/10.3892/or.2012.2033