Expression of AEG-1 in human T-cell lymphoma enhances the risk of progression

  • Authors:
    • Jiaqin Yan
    • Mingzhi Zhang
    • Qingjiang Chen
    • Xudong Zhang
  • View Affiliations

  • Published online on: September 26, 2012     https://doi.org/10.3892/or.2012.2055
  • Pages: 2107-2114
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Abstract

The aim of this study was to examine the expression and role of astrocyte elevated gene-1 (AEG-1) in biological processes of T-cell non-Hodgkin's lymphoma (T-NHL). AEG-1 expression in T-NHL patients was characterized with immunohistochemistry. The expression of AEG-1, survivin, Bcl-2 and Bax in Jurkat and Hut-78 cells was detected by real-time PCR and western blotting. Cell proliferation, cell cycle and apoptosis were measured by MTT and flow cytometry. MMP-2/-9 activity was detected by gelatin zymography. Of the studied tumors, 104 (80.62%) exhibited cytoplasmic AEG-1 immunostaining. AEG-1-siRNA in Jurkat and Hut-78 cells suppressed cell proliferation and induced cell apoptosis, inhibited survivin and Bcl-2/Bax protein expression as well as MMP-2/-9 activity. Downregulation of AEG-1 using siRNA could provide a potential approach for gene therapy against T-NHL, and the antitumor effects may be associated with inhibition of survivin and Bcl-2/Bax protein expression and MMP-2/-9 activity.
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December 2012
Volume 28 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yan J, Zhang M, Chen Q and Zhang X: Expression of AEG-1 in human T-cell lymphoma enhances the risk of progression. Oncol Rep 28: 2107-2114, 2012.
APA
Yan, J., Zhang, M., Chen, Q., & Zhang, X. (2012). Expression of AEG-1 in human T-cell lymphoma enhances the risk of progression. Oncology Reports, 28, 2107-2114. https://doi.org/10.3892/or.2012.2055
MLA
Yan, J., Zhang, M., Chen, Q., Zhang, X."Expression of AEG-1 in human T-cell lymphoma enhances the risk of progression". Oncology Reports 28.6 (2012): 2107-2114.
Chicago
Yan, J., Zhang, M., Chen, Q., Zhang, X."Expression of AEG-1 in human T-cell lymphoma enhances the risk of progression". Oncology Reports 28, no. 6 (2012): 2107-2114. https://doi.org/10.3892/or.2012.2055