microRNA-181a is associated with poor prognosis of colorectal cancer

Nishimura,Junichi; Handa,Rio; Yamamoto,Hirofumi; Tanaka,Fumiaki; Shibata,Kohei; Mimori,Koshi; Takemasa,Ichiro; Mizushima,Tsunekazu; Ikeda,Masataka; Sekimoto,Mitsugu; Ishii,Hideshi; Doki,Yuichiro; Mori,Masaki

doi:10.3892/or.2012.2059

microRNA-181a is associated with poor prognosis of colorectal cancer

Authors:
- Junichi Nishimura
- Rio Handa
- Hirofumi Yamamoto
- Fumiaki Tanaka
- Kohei Shibata
- Koshi Mimori
- Ichiro Takemasa
- Tsunekazu Mizushima
- Masataka Ikeda
- Mitsugu Sekimoto
- Hideshi Ishii
- Yuichiro Doki
- Masaki Mori
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Affiliations: Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan, Department of Molecular and Cellular Biology, Division of Molecular and Surgical Oncology, Medical Institute of Bioregulation, Kyushu University, Beppu, Ohita 874-0838, Japan
Published online on: September 26, 2012 https://doi.org/10.3892/or.2012.2059
Pages: 2221-2226

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Abstract

miRNAs regulate gene expression at the post-transcriptional level by degradation of mRNA and translational repression. Recent studies have shown that miR-181a is dysregulated in several types of cancer; however, the clinical significance of miR‑181a in colorectal cancer (CRC) remains unclear. We addressed this question by using quantitative real-time PCR (qRT-PCR) to analyze miR-181a expression in 162 CRC patients. There was no significant difference in miR-181a expression in normal colon vs. colorectal cancer tissue. The cancer tissue samples were categorized into a low and high expression group based on miR-181a expression. Comparison of the clinicopathological factors and prognosis in these two groups showed that the high expression group had a significantly poorer prognosis than the low expression group (P=0.011). Multivariate analysis indicated that high miR-181a expression was an independent significant prognostic factor for CRC. However, there no correlation was observed between miR-181a expression and clinicopathological parameters. In vitro analysis revealed that the overexpression of miR-181a repressed the expression of the tumor suppressor, phosphatase and tensin homolog (PTEN) located on chromosome 10, at the mRNA level. These data suggest that miR-181a may be a new independent prognostic factor for CRC patients.

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