The effect of G-CSF on F-actin reorganization in HL-60 and K562 cell lines

Izdebska,Magdalena; Grzanka,Dariusz; Gagat,Maciej; Gackowska,Lidia; Grzanka,Alina

doi:10.3892/or.2012.2061

The effect of G-CSF on F-actin reorganization in HL-60 and K562 cell lines

Authors:
- Magdalena Izdebska
- Dariusz Grzanka
- Maciej Gagat
- Lidia Gackowska
- Alina Grzanka
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Affiliations: Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-092 Bydgoszcz, Poland, Department of Clinical Pathomorphology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-092 Bydgoszcz, Poland, Department of Immunology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-092 Bydgoszcz, Poland
Published online on: September 26, 2012 https://doi.org/10.3892/or.2012.2061
Pages: 2138-2148

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Abstract

The aim of this investigation was to show the influence of G-CSF (G-CSF) on the F-actin cytoskeleton and the morphology of G-CSFR-proficient HL-60 and G-CSFR-deficient K562 cell lines. In the present study, we show changes in F-actin distribution in HL-60 cells after treatment with 5 and 10 ng/ml concentration of G-CSF but also changes in the organization and fluorescence intensity of F-actin in the K562 cell line. After treatment of HL-60 cells with 5 ng/ml concentration of G-CSF we observed an increase in F-actin levels. Additionally, a higher labeling of nuclear F-actin under TEM was observed. Moreover, changes in the cell cycle indicate cell differentiation. On the other hand, in the K562 cell line we observed an increase in the percentage sub-G1 cells following treatment with both concentration of G-CSF. Furthermore, an increase in the percentage of late apoptotic cells after G-CSF treatment was observed. A statistically significant difference in the cytoplasmic F-actin levels was not detected, but nuclear levels were decreased. In conclusion, we suggest that the G-CSF-based reorganization of actin filaments in HL-60 cells is involved in the differentiation process. Moreover, we suggest that the G-CSF-induced changes observed in K562 cells are associated with a G-CSF receptor-independent pathway or its binding to other similar receptors.

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1	Oppenheim JJ: Cytokines: past, present and future. Int J Hematol. 74:3–8. 2001. View Article : Google Scholar : PubMed/NCBI
2	Basu S, Dunn A and Ward A: G-CSF: function and modes of action. Int J Mol Med. 10:3–10. 2002.PubMed/NCBI
3	Beekman R and Touw IP: G-CSF and its receptor in myeloid malignancy. Blood. 115:5131–5136. 2010. View Article : Google Scholar : PubMed/NCBI
4	Franzke A: The role of G-CSF in adaptive immunity. Cytokine Growth Factor Rev. 17:235–244. 2006. View Article : Google Scholar : PubMed/NCBI
5	Leavey PJ, Sellins KS, Thurman G, et al: In vivo treatment with granulocyte colony-stimulating factor results in divergent effects on neutrophil functions measured in vitro. Blood. 92:4366–4374. 1998.PubMed/NCBI
6	Suzuki S, Kobayashi M, Chiba K, et al: Autocrine production of epithelial cell-derived neutrophil attractant-78 induced by granulocyte colony-stimulating factor in neutrophils. Blood. 99:1863–1865. 2002.PubMed/NCBI
7	Bradstock KF: The use of hematopoietic growth factors in the treatment of acute leukemia. Curr Pharm Des. 8:343–355. 2002. View Article : Google Scholar : PubMed/NCBI
8	Carulli G, Mattii L, Azzara A, et al: Actin polymerization in neutrophils from donors of peripheral blood stem cells: divergent effects of glycosylated and nonglycosylated recombinant human granulocyte colony-stimulating factor. Am J Hematol. 81:318–323. 2006. View Article : Google Scholar
9	Håkansson L, Höglund M, Jönsson UB, Torsteinsdottir I, Xu X and Venge P: Effects of in vivo administration of G-CSF on neutrophil and eosinophil adhesion. Br J Haematol. 98:603–611. 1997.PubMed/NCBI
10	Ripa RS and Kastrup J: G-CSF therapy with mobilization of bone marrow stem cells for myocardial recovery after acute myocardial infarction - a relevant treatment? Exp Hematol. 36:681–686. 2008. View Article : Google Scholar : PubMed/NCBI
11	Ku NO, Zhou X, Toivola DM and Omary MB: The cytoskeleton of digestive epithelia in health and disease. Am J Physiol. 277:G1108–G1137. 1999.PubMed/NCBI
12	Bursch W, Hochegger K, Torok L, Marian B, Ellinger A and Hermann RS: Autophagic and apoptotic types of programmed cell death exhibit different fates of cytoskeletal filaments. J Cell Sci. 113:1189–1198. 2000.PubMed/NCBI
13	Pollard TD: The cytoskeleton, cellular motility and the reductionist agenda. Nature. 422:741–745. 2003. View Article : Google Scholar : PubMed/NCBI
14	Ndozangue-Touriguine O, Hamelin J and Bréard J: Cytoskeleton and apoptosis. Biochem Pharmacol. 76:11–18. 2008. View Article : Google Scholar
15	Hightower RC and Meagher RB: The molecular evolution of actin. Genetics. 114:315–332. 1986.PubMed/NCBI
16	Akisaka T, Yoshida H, Inoue S and Shimizu K: Organization of cytoskeletal F-actin, G-actin, and gelsolin in the adhesion structures in cultured osteoclast. J Bone Miner Res. 16:1248–1255. 2001. View Article : Google Scholar : PubMed/NCBI
17	White S, Williams P, Wojcik KR, Sun S, Hiemstra PS, Rabe KF and Dorscheid DR: Initiation of apoptosis by actin cytoskeletal derangement in human airway epithelial cells. Am J Respir Cell Mol Biol. 24:282–294. 2001. View Article : Google Scholar : PubMed/NCBI
18	Ascough KR: Endocytosis: Actin in the driving seat. Curr Biol. 14:R124–R126. 2004. View Article : Google Scholar : PubMed/NCBI
19	Carlier MF and Pantaloni D: Control of actin assembly dynamics in cell motility. J Biol Chem. 282:23005–23009. 2007. View Article : Google Scholar : PubMed/NCBI
20	Pederson T and Aebi U: Actin in the nucleus: what form and what for? J Struct Biol. 140:3–9. 2002. View Article : Google Scholar : PubMed/NCBI
21	Visa N and Percipalle P: Nuclear functions of actin. Cold Spring Harb Perspect Biol. 2:a0006202010. View Article : Google Scholar : PubMed/NCBI
22	Olave IA, Reck-Peterson SL and Crabtree GR: Nuclear actin and actin-related proteins in chromatin remodeling. Annu Rev Biochem. 71:755–781. 2002. View Article : Google Scholar : PubMed/NCBI
23	Blessing CA, Ugrinova GT and Goodson HV: Actin and ARPs: action in the nucleus. Trends Cell Biol. 14:435–442. 2004. View Article : Google Scholar : PubMed/NCBI
24	Hofmann WA, Stojiljkovic L, Fuchsova B, et al: Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II. Nat Cell Biol. 6:1094–1101. 2004. View Article : Google Scholar : PubMed/NCBI
25	Miralles F and Visa N: Actin in transcription and transcription regulation. Curr Opin Cell Biol. 18:261–266. 2006. View Article : Google Scholar : PubMed/NCBI
26	Chen M and Shen X: Nuclear actin and actin-related proteins in chromatin dynamics. Curr Opin Cell Biol. 19:326–330. 2007. View Article : Google Scholar : PubMed/NCBI
27	Pederson T: As functional nuclear actin comes into view, is it globular, filamentous, or both? J Cell Biol. 180:1061–1064. 2008. View Article : Google Scholar : PubMed/NCBI
28	Gieni RS and Hendzel MJ: Actin dynamics and functions in the interphase nucleus: moving toward an understanding of nuclear polymeric actin. Biochem Cell Biol. 87:283–306. 2009.PubMed/NCBI
29	Xu YZ, Thuraisingam T, Morais DA, Rola-Pleszczynski M and Radzioch D: Nuclear translocation of β-actin is involved in transcriptional regulation during macrophage differentiation of HL-60 cells. Mol Biol Cell. 21:811–820. 2010.
30	Kutsuna H, Suzuki K, Kamata N, et al: Actin reorganization and morphological changes in human neutrophils stimulated by TNF, GM-CSF and G-CSF: the role of MAP kinases. Am J Physiol Cell Physiol. 286:55–64. 2004. View Article : Google Scholar : PubMed/NCBI
31	El-Sonbaty SS, Watanabe M, Hochito K, Yamaguchi K, Matsuda I and Tsuchiya H: Exogenously expressed granulocyte colony-stimulating factor (G-CSF) receptor on K562 cells can transduce G-CSF-triggered growth and differentiation signals. Int J Hematol. 61:61–68. 1995. View Article : Google Scholar
32	Saito K, Nakamura Y, Waga K, et al: Mature and immature myeloid cells decrease the granulocyte colony-stimulating factor level by absorption of granulocyte colony-stimulating factor. Int J Hematol. 67:145–151. 1998. View Article : Google Scholar : PubMed/NCBI
33	Brandstetter T, Ninci E, Bettendorf H, et al: Granulocyte colony stimulating factor (G-CSF) receptor gene expression of ovarian carcinoma does not correlate with G-CSF caused cell proliferation. Cancer. 91:1372–1383. 2001. View Article : Google Scholar : PubMed/NCBI
34	Veselska R, Zitterbart K, Jelinkova S, Neradil J and Svoboda A: Specific cytoskeleton changes during apoptosis accompanying induced differentiation of HL-60 myeloid leukemia cells. Oncol Rep. 10:1049–1058. 2003.
35	Godyn JJ, Xu H, Zhang F, Kolla S and Studzinski GP: A dual block to cell cycle progression in HL60 cells exposed to analogues of vitamin D3. Cell Prolif. 27:37–46. 1994. View Article : Google Scholar : PubMed/NCBI
36	Erenpreisa J and Cragg MS: Mitotic death: a mechanism of survival? Cancer Cell Int. 1:12001. View Article : Google Scholar
37	Zitterbart K and Veselska R: Effect of retinoic acid on the actin cytoskeleton in HL-60 cells. Neoplasma. 48:456–461. 2001.PubMed/NCBI
38	Grzanka A, Grzanka D and Orlikowska M: Cytoskeletal reorganization during process of apoptosis induced by cytostatic drugs in K-562 and HL-60 leukemia cell lines. Biochem Pharmacol. 66:1611–1617. 2003. View Article : Google Scholar : PubMed/NCBI
39	Atencia R, Garcia-Sanz M, Perez-Yarza G, Asumendi A, Hilario E and Arechaga J: A structural analysis of cytoskeleton components during the execution chase of apoptosis. Protoplasma. 198:163–169. 1997. View Article : Google Scholar
40	Brown SB, Bailey K and Savill J: Actin is cleaved during constitutive apoptosis. Biochem J. 323:233–237. 1997.PubMed/NCBI
41	Grzanka A, Izdebska M, Litwiniec A, Grzanka D and Safiejko-Mroczka B: Actin filament reorganization in HL-60 leukemia cell line after treatment with G-CSF and GM-CSF. Folia Histochem Cytobiol. 45:191–197. 2007.PubMed/NCBI
42	Chodniewicz D and Zhelev DV: Novel pathway of F-actin polymerization in the human neutrophil. Blood. 102:2251–2258. 2003. View Article : Google Scholar : PubMed/NCBI
43	Gomez-Cambronero J, Horn J, Paul CC and Baumann MA: Granulocyte-macrophage colony-stimulating factor is a chemoattractant cytokine for human neutrophils: involvement of the ribosomal p70 S6 kinase signaling pathway. J Immunol. 171:6846–6855. 2003. View Article : Google Scholar
44	Mailänder V and Landfester K: Interaction of nanoparticles with cells. Biomacromolecules. 10:2379–2400. 2009.
45	Izdebska M, Grzanka D, Gackowska L, Żuryń A and Grzanka A: The influence of Trisenox on action organization in HL-60 cells. Cent Eur J Biol. 4:351–361. 2009. View Article : Google Scholar
46	Grzanka D, Marszałek A, Gagat M, Izdebska M, Gackowska L and Grzanka A: Doxorubicin-induced F-actin reorganization in cofilin-1 (nonmuscle) down-regulated CHO AA8 cells. Folia Histochem Cytobiol. 48:377–386. 2010. View Article : Google Scholar : PubMed/NCBI
47	Izdebska M, Grzanka A, Ostrowski M, Żuryń A and Grzanka D: Effect of arsenic trioxide (Trisenox) on actin organization in K-562 erythroleukemia cells. Folia Histochem Cytobiol. 47:453–459. 2009.PubMed/NCBI
48	Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A and Crabtree GR: Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling. Cell. 95:625–636. 1998. View Article : Google Scholar : PubMed/NCBI
49	Widlak P, Palyvoda O, Kumala S and Garrard WT: Modeling apoptotic chromatin condensation in normal cell nuclei. Requirement for intranuclear mobility and actin involvement. J Biol Chem. 277:21683–21690. 2002. View Article : Google Scholar : PubMed/NCBI
50	Sjolinder M, Bjork P, Soderberg E, Sabri N, Farrants AK and Visa N: The growing pre-mRNA recruits actin and chromatin-modifying factors to transcriptionally active genes. Genes Dev. 19:1871–1884. 2005. View Article : Google Scholar : PubMed/NCBI
51	Zhu QS, Xia L, Mills GB, Lowell CA, Touw IP and Corey SJ: G-CSF induced reactive oxygen species involves Lyn-PI3-kinase-Akt and contributes to myeloid cell growth. Blood. 107:1847–1856. 2006. View Article : Google Scholar : PubMed/NCBI
52	Kitagawa J, Hara T, Tsurumi H, Kanemura N, Kasahara S, Shimizu M and Moriwaki H: Cell cycle-dependent priming action of granulocyte colony-stimulating factor (G-CSF) enhances in vitro apoptosis induction by cytarabine and etoposide in leukemia cell lines. J Clin Exp Hematop. 50:99–105. 2010. View Article : Google Scholar
53	Chow DC, Brevnova L, He XL, Martick MM, Bankovich A and Garcia KC: A structural template for gp130-cytokine signaling assemblies. Biochim Biophys Acta. 1592:225–235. 2002. View Article : Google Scholar : PubMed/NCBI
54	Gearing DP, Thut CJ, VandeBos T, et al: Leukemia inhibitory factor receptor is structurally related to the IL-6 signal transducer, gp130. EMBO J. 10:2839–2848. 1991.PubMed/NCBI
55	Tartaglia LA, Dembski M, Weng X, et al: Identification and expression cloning of a leptin receptor, OB-R. Cell. 83:1263–1271. 1995. View Article : Google Scholar : PubMed/NCBI