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Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features

  • Authors:
    • Yifeng Sun
    • Bo Su
    • Peng Zhang
    • Huikang Xie
    • Hui Zheng
    • Yongjie Xu
    • Qiaoling Du
    • Huan Zeng
    • Xiao Zhou
    • Chang Chen
    • Wen Gao

  • View Affiliations

  • Published online on: November 28, 2012     https://doi.org/10.3892/or.2012.2152
  • Pages: 704-712
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Abstract

The present study investigated the expression of miR-150 and miR-3940-5p in non-small cell lung carcinoma (NSCLC) and its relationship with clinicopathologic features. Samples included tumor, tumor-adjacent and normal lung parenchyma tissues from 90 NSCLC patients and 17 cases of embryonic lung cDNA. The expression levels of miR-150, miR-18b-5p, miR-643 and miR-3940-5p were detected by real‑time PCR; p53, EGFR, Kras and Ki-67 expression in tumor tissues was determined by immunohistochemistry. p53 mRNA expression levels in NSCLC were examined by SYBR-Green real-time PCR. The relationship between the four miRNAs and clinicopathologic features of 90 cases was analyzed. The expression of miR-150 and miR-3940-5p was significantly downregulated in tumor tissues and embryonic lung tissues compared to normal lung tissues. The expression of miR-150 and miR-3940-5p in tumor tissues was also lower than that in the matched tumor-adjacent tissues. miR-150 was downregulated preferentially in subgroups of patients with a tumor diameter more than or equal to 3 cm, in smokers and in stage III and IV tumors. Specifically, miR-150 and miR-3940-5p expression was decreased in nuclear cell proliferation antigen Ki-67-positive NSCLC cases. miR-150 and miR-3940-5p were found to be significantly downregulated in p53 IHC-positive NSCLC cases and were negatively correlated with p53 mRNA. Reduced miR-150 and miR-3940-5p expression in tumor tissues and embryonic lung tissues suggests that these miRs may be involved in the tumorigenesis or de-differentiation of NSCLC. Due to this associaton with the Ki-67 proliferation index in NSCLC, downregulation of miR-150 and miR-3940-5p may contribute to tumor growth and proliferation. miR-150 and miR-3940-5p may affect p53 expression through a direct or indirect pathway.
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February 2013
Volume 29 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Impact Factor: 3.8
  • Ranked Oncology
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  • CiteScore: 8.5
  • CiteScore Rank: Q1: #74/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
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Spandidos Publications style
Sun Y, Su B, Zhang P, Xie H, Zheng H, Xu Y, Du Q, Zeng H, Zhou X, Chen C, Chen C, et al: Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features. Oncol Rep 29: 704-712, 2013.
APA
Sun, Y., Su, B., Zhang, P., Xie, H., Zheng, H., Xu, Y. ... Gao, W. (2013). Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features. Oncology Reports, 29, 704-712. https://doi.org/10.3892/or.2012.2152
MLA
Sun, Y., Su, B., Zhang, P., Xie, H., Zheng, H., Xu, Y., Du, Q., Zeng, H., Zhou, X., Chen, C., Gao, W."Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features". Oncology Reports 29.2 (2013): 704-712.
Chicago
Sun, Y., Su, B., Zhang, P., Xie, H., Zheng, H., Xu, Y., Du, Q., Zeng, H., Zhou, X., Chen, C., Gao, W."Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung carcinoma and correlates with clinicopathological features". Oncology Reports 29, no. 2 (2013): 704-712. https://doi.org/10.3892/or.2012.2152