Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells

  • Authors:
    • Soo-Jin Oh
    • Seung Pil Jung
    • Jeonghun Han
    • Sangmin Kim
    • Jee Soo Kim
    • Seok Jin Nam
    • Jeong Eon Lee
    • Jung-Han Kim
  • View Affiliations

  • Published online on: January 24, 2013     https://doi.org/10.3892/or.2013.2252
  • Pages: 1343-1348
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Abstract

Matrix metalloproteinases (MMPs) play an important role in cancer metastasis, cell migration and invasion. Herein, we investigated the effects of silibinin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Our results revealed that the levels of MMP-9 mRNA and protein expression were significantly increased by TPA but not MMP-2 in TPC-1 and MCF7 cells. To verify the regulatory mechanism of TPA-induced MMP-9 expression, we treated TPC-1 and MCF7 cells with the MEK1/2 inhibitor, UO126, and TPA-induced MMP-9 expression was significantly decreased. We also found that TPA-induced cell migration and MMP-9 expression was significantly decreased by silibinin. In addition, TPA-induced phosphorylation of MEK and ERK was also inhibited by silibinin. Taken together, we suggest that silibinin suppresses TPA-induced cell migration and MMP-9 expression through the MEK/ERK-dependent pathway in thyroid and breast cancer cells.
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April 2013
Volume 29 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Oh S, Jung SP, Han J, Kim S, Kim JS, Nam SJ, Lee JE and Kim J: Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Oncol Rep 29: 1343-1348, 2013.
APA
Oh, S., Jung, S.P., Han, J., Kim, S., Kim, J.S., Nam, S.J. ... Kim, J. (2013). Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Oncology Reports, 29, 1343-1348. https://doi.org/10.3892/or.2013.2252
MLA
Oh, S., Jung, S. P., Han, J., Kim, S., Kim, J. S., Nam, S. J., Lee, J. E., Kim, J."Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells". Oncology Reports 29.4 (2013): 1343-1348.
Chicago
Oh, S., Jung, S. P., Han, J., Kim, S., Kim, J. S., Nam, S. J., Lee, J. E., Kim, J."Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells". Oncology Reports 29, no. 4 (2013): 1343-1348. https://doi.org/10.3892/or.2013.2252