miR-101 inhibits autophagy and enhances cisplatin-induced apoptosis in hepatocellular carcinoma cells

Xu,Yonghua; An,Yong; Wang,Yun; Zhang,Changhe; Zhang,Hai; Huang,Changjun; Jiang,Hao; Wang,Xuehao; Li,Xiangcheng

doi:10.3892/or.2013.2338

miR-101 inhibits autophagy and enhances cisplatin-induced apoptosis in hepatocellular carcinoma cells

Authors:
- Yonghua Xu
- Yong An
- Yun Wang
- Changhe Zhang
- Hai Zhang
- Changjun Huang
- Hao Jiang
- Xuehao Wang
- Xiangcheng Li
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Affiliations: Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Nanjing, Jiangsu 210029, P.R. China
Published online on: March 7, 2013 https://doi.org/10.3892/or.2013.2338
Pages: 2019-2024

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Abstract

Hepatocellular carcinoma (HCC) ranks third in cancer-related mortality due to late diagnosis and poor treatment options. Autophagy is a lysosome-mediated protein and organelle degradation process which is characterized by the formation of double-membrane vesicles, known as autophagosomes. Increasing evidence reveals that autophagy functions as a survival mechanism in liver cancer cells against drug-induced apoptosis. In this study, we found that autophagy was suppressed by miR-101 in the HCC cell line HepG2. miR-101 inhibited autophagy via targets including RAB5A, STMN1 and ATG4D. Moreover, miR-101 enhanced apoptosis induced by cisplatin in the HepG2 cell line. The possible mechanism of this effect may be through inhibition of autophagy. Our results indicate a novel and critical role for miR-101 and autophagy in the chemoresistance of cisplatin in HCC. We propose that gene therapy targeting miR-101/autophagy should be investigated further as a potential alternative therapeutic strategy for HCC.

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