Downregulation of the GnT-V gene inhibits metastasis and invasion of BGC823 gastric cancer cells

  • Authors:
    • Binbin Huang
    • Longe Sun
    • Jianchun Cao
    • Yunyun Zhang
    • Qiong Wu
    • Junjie Zhang
    • Yanli Ge
    • Liu Fu
    • Zhirong Wang
  • View Affiliations

  • Published online on: April 2, 2013     https://doi.org/10.3892/or.2013.2373
  • Pages: 2392-2400
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Abstract

Metastatic and invasive potential is a barrier to the successful treatment of gastric cancer. N-acetylgluco­saminyltransferase V (GnT-V), a key enzyme catalyzing the formation of 1,6 N-acetylglucosamine (GlcNAc), has been demonstrated to display a distinct function in different types of tumors. The aim of this study was to investigate the role of GnT-V in the invasive potential of BGC823 human gastric cancer cells in vitro and the possible underlying mechanism. GnT-V was downregulated in BGC823 cells by oligo-siRNA transfection. Cell proliferation and invasiveness were assessed by CCK-8 assay, Tunel assay, scratch-wound assay as well as transwell assay. The products of GnT-V, β1-6 branching of asparagine-linked oligosaccharides, were determined by L-PHA lectin blot analysis. The expression of EGFRs, E-cadherin/vimentin and MMP-2/MMP-9 was analyzed both at the mRNA and protein levels. The results showed that downregulation of GnT-V decreased proliferation and the metastatic/invasive potential of BGC823 cells. The expression of EGFRs, E-cadherin/vimentin and MMP-9, molecules related to cancer metastasis and invasion in various tumors, were influenced correspondingly. These findings suggest that downregulation of GnT-V inhibited cell metastasis and invasion of BGC823 cells via EGFR signaling-initiated EMT phenotype and MMP-9 expression. These results provide a novel mechanism to explain the role of GnT-V in cell metastasis and invasion.
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June 2013
Volume 29 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Huang B, Sun L, Cao J, Zhang Y, Wu Q, Zhang J, Ge Y, Fu L and Wang Z: Downregulation of the GnT-V gene inhibits metastasis and invasion of BGC823 gastric cancer cells . Oncol Rep 29: 2392-2400, 2013.
APA
Huang, B., Sun, L., Cao, J., Zhang, Y., Wu, Q., Zhang, J. ... Wang, Z. (2013). Downregulation of the GnT-V gene inhibits metastasis and invasion of BGC823 gastric cancer cells . Oncology Reports, 29, 2392-2400. https://doi.org/10.3892/or.2013.2373
MLA
Huang, B., Sun, L., Cao, J., Zhang, Y., Wu, Q., Zhang, J., Ge, Y., Fu, L., Wang, Z."Downregulation of the GnT-V gene inhibits metastasis and invasion of BGC823 gastric cancer cells ". Oncology Reports 29.6 (2013): 2392-2400.
Chicago
Huang, B., Sun, L., Cao, J., Zhang, Y., Wu, Q., Zhang, J., Ge, Y., Fu, L., Wang, Z."Downregulation of the GnT-V gene inhibits metastasis and invasion of BGC823 gastric cancer cells ". Oncology Reports 29, no. 6 (2013): 2392-2400. https://doi.org/10.3892/or.2013.2373