Specific reversal of tumor-suppressor gene promoter hypermethylation with bovine oocyte extract

  • Authors:
    • Zhenfei Wang
    • Haixia Gao
    • Huimin Wang
    • Xiaolu Ren
    • Luri Bao
    • Rula Sa
    • Jie Wang
    • Haidong Bai
    • Haiquan Yu
  • View Affiliations

  • Published online on: May 10, 2013     https://doi.org/10.3892/or.2013.2449
  • Pages: 179-184
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Abstract

Epigenetic silencing of tumor-suppressor genes by promoter hypermethylation contributes considerably to the initiation and progression of cancer. Nucleoside analogs, the most widely used DNA methylation inhibitors, have the drawbacks of inducing repetitive sequence hypomethylation. Here, we aimed to specifically reverse tumor-suppressor gene (TSG) promoter hypermethylation with bovine oocyte extract. H460 human lung cancer cells were reversibly permeabilized and incubated with bovine oocyte extract for 3.5 h. The extract treatment led to significant demethylation of the hypermethylated promoters of the TSGs RUNX3, CDH1, RASSF1A and WIF1; however, the methylation levels of repetitive sequences were not affected. The promoter demethylation induced by bovine oocyte extract substantially upregulated the expression of RUNX3, CDH1, RASSF1A and WIF1, and significantly inhibited the anchorage-independent proliferation, migration and invasion of H460 cells. This study demonstrates that bovine oocyte extract can reverse the malignant phenotype by serving as an efficient and safe DNA demethylator. The active demethylation activity of bovine oocyte extract is valuable for dissecting the epigenetic alterations in cancer cells and developing novel safe anticancer drugs based on epigenetic mechanisms.
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July 2013
Volume 30 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang Z, Gao H, Wang H, Ren X, Bao L, Sa R, Wang J, Bai H and Yu H: Specific reversal of tumor-suppressor gene promoter hypermethylation with bovine oocyte extract. Oncol Rep 30: 179-184, 2013.
APA
Wang, Z., Gao, H., Wang, H., Ren, X., Bao, L., Sa, R. ... Yu, H. (2013). Specific reversal of tumor-suppressor gene promoter hypermethylation with bovine oocyte extract. Oncology Reports, 30, 179-184. https://doi.org/10.3892/or.2013.2449
MLA
Wang, Z., Gao, H., Wang, H., Ren, X., Bao, L., Sa, R., Wang, J., Bai, H., Yu, H."Specific reversal of tumor-suppressor gene promoter hypermethylation with bovine oocyte extract". Oncology Reports 30.1 (2013): 179-184.
Chicago
Wang, Z., Gao, H., Wang, H., Ren, X., Bao, L., Sa, R., Wang, J., Bai, H., Yu, H."Specific reversal of tumor-suppressor gene promoter hypermethylation with bovine oocyte extract". Oncology Reports 30, no. 1 (2013): 179-184. https://doi.org/10.3892/or.2013.2449