search button

miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis

  • Authors:
    • De-Tao Wang
    • Zhong-Liang Ma
    • Yan-Li Li
    • Yue-Qing Wang
    • Bo-Tao Zhao
    • Jia-Li Wei
    • Xiang Qi
    • Xin-Tai Zhao
    • You-Xin Jin

  • View Affiliations

  • Published online on: May 13, 2013     https://doi.org/10.3892/or.2013.2453
  • Pages: 492-498
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

microRNAs (miRNAs) are a class of non-coding small RNAs that act as negative regulators of gene expression by binding to the 3'-untranslated region (3'-UTR) of target mRNAs. Tumor protein p53, a transcriptional factor, plays an important role in the progression of tumorigenesis. miR-150 was the only miRNA predicted to target 3'-UTR of p53 by Targetscan. In order to investigate the function of miR-150, p53 and relevant miRNAs in non-small cell lung cancer (NSCLC), we constructed two expression vectors of p53 (pcDNA3.1-p53 and pcDNA3.1-p53-3'-UTR) and two report vectors (pGL3-p53-3'-UTR and pGL3-p53-3'-mUTR). The activity of luciferase transfected with miR-150 mimics was lower by 30% when compared to that of the miRNA-negative control (miRNA-NC). Moreover, the p53 protein was downregulated by at least 50% when miR-150 mimics were cotransfected with pcDNA3.1-p53-3'-UTR when compared to miRNA-NC. We also determined the expression of miR-150 and p53 in NSCLC patient tissue samples. The expression of miR-150 in T2 stage tissue samples was higher than that in T1 stage tissue samples. The corresponding target gene p53 was correlated with miR-150 expression. In the present study, we further analyzed the cell cycle distribution. The cells transfected with pcDNA3.1-p53 were significantly arrested in the G1 phase when compared to the control cells. When miR-150 mimics were cotransfected with pcDNA3.1-p53-3'-UTR, the percentage of cells in the G1 phase was significantly lower by 4% when compared to miRNA-NC. To identify miRNAs that are regulated by the p53 protein, qRT-PCR was performed after pcDNA3.1-p53 transfection. miR-34a, miR-184, miR-181a and miR-148 were upregulated significantly. However, there was no distinct difference in the expression of miR-10a, miR-182 and miR-34c. Our results showed that miR-150 targets the 3'-UTR of p53, and p53 protein promotes the expression of miRNAs which affect cell cycle progression. These findings suggest that miR-150, p53 protein and relevant miRNAs are members of a regulatory network in NSCLC tumorigenesis.
View Figures
View References

Related Articles

Journal Cover

July 2013
Volume 30 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 3.8
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 8.5
  • CiteScore Rank: Q1: #74/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Ma Z, Li Y, Wang Y, Zhao B, Wei J, Qi X, Zhao X and Jin Y: miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis. Oncol Rep 30: 492-498, 2013.
APA
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J. ... Jin, Y. (2013). miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis. Oncology Reports, 30, 492-498. https://doi.org/10.3892/or.2013.2453
MLA
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J., Qi, X., Zhao, X., Jin, Y."miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis". Oncology Reports 30.1 (2013): 492-498.
Chicago
Wang, D., Ma, Z., Li, Y., Wang, Y., Zhao, B., Wei, J., Qi, X., Zhao, X., Jin, Y."miR-150, p53 protein and relevant miRNAs consist of a regulatory network in NSCLC tumorigenesis". Oncology Reports 30, no. 1 (2013): 492-498. https://doi.org/10.3892/or.2013.2453