Melanoma antigen gene family A as a molecular marker of gastric and colorectal cancers

  • Authors:
    • Tae-Bum Lee
    • Sung-Chul Lim
    • Young-Sook Moon
    • Cheol-Hee Choi
  • View Affiliations

  • Published online on: May 14, 2013     https://doi.org/10.3892/or.2013.2458
  • Pages: 234-238
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Abstract

The present study aimed to evaluate the role of melanoma antigen family A (MAGEA) in gastric and colorectal cancer cell lines and clinical tissue samples. we used 10 gastric and 9 colorectal cancer cell lines, 20 early-stage and 21 advanced-stage gastric cancer tissues, 20 colon adenomas and 19 colorectal cancer tissues. Real-time RT-PCR assay was used for the determination of MAGEA mRNA levels. Western blot analysis and immunohistochemistry were used for the determination of MAGEA protein levels in cell lines and tissues, respectively. Gastric and colorectal cancer cell lines showed variable mRNA expression levels of MAGEA. The MAGEA protein was detected in 30% of gastric cancer cell lines and in 22.2% of colorectal cancer cell lines. There was a high correlation between mRNA and protein expression. Regarding the clinical samples, MAGEA expression was noted in 25, 28.6 and 31.6%, respectively in early-stage, advanced-stage gastric cancer tissues and colon adenocarcinoma, but was negative in the adjacent normal tissues of the stomach and colon as well as colon adenoma. These results indicate that MAGEA is involved in the carcinogenesis of gastric and colorectal cancer and, therefore, can be used as a diagnostic marker to predict these cancers.
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July 2013
Volume 30 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lee T, Lim S, Moon Y and Choi C: Melanoma antigen gene family A as a molecular marker of gastric and colorectal cancers. Oncol Rep 30: 234-238, 2013.
APA
Lee, T., Lim, S., Moon, Y., & Choi, C. (2013). Melanoma antigen gene family A as a molecular marker of gastric and colorectal cancers. Oncology Reports, 30, 234-238. https://doi.org/10.3892/or.2013.2458
MLA
Lee, T., Lim, S., Moon, Y., Choi, C."Melanoma antigen gene family A as a molecular marker of gastric and colorectal cancers". Oncology Reports 30.1 (2013): 234-238.
Chicago
Lee, T., Lim, S., Moon, Y., Choi, C."Melanoma antigen gene family A as a molecular marker of gastric and colorectal cancers". Oncology Reports 30, no. 1 (2013): 234-238. https://doi.org/10.3892/or.2013.2458