Molecular characteristics of homologous salivary adenoid cystic carcinoma cell lines with different lung metastasis ability

Wang,Lin; Wang,Yixiang; Bian,Huan; Pu,Yinfei; Guo,Chuanbin

doi:10.3892/or.2013.2460

Molecular characteristics of homologous salivary adenoid cystic carcinoma cell lines with different lung metastasis ability

Authors:
- Lin Wang
- Yixiang Wang
- Huan Bian
- Yinfei Pu
- Chuanbin Guo
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Affiliations: Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, P.R. China, Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, P.R. China
Published online on: May 14, 2013 https://doi.org/10.3892/or.2013.2460
Pages: 207-212

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Abstract

Although the homologous salivary adenoid cystic carcinoma (SACC) cell lines SACC-83 and SACC-LM have already been used as SACC models to investigate the underlying mechanisms of metastasis, the molecular features of these SACC cell lines remain unclear. We screened 136 genes related to metastasis in order to investigate the biological and molecular properties of these two cell lines by short tandem repeat (STR) profiling, immunostaining, transwell invasion assay, real-time PCR and western blotting. STR and immunostaining results showed that SACC-83 and SACC-LM are homologous cancer cell lines, derived from adenoepithelial cells and, to date, are not contaminated by each other or other cancer cell lines. Transwell invasion assay results showed that SACC-LM had increased invasion ability compared to SACC‑83. 29 of the 136 differentially expressed genes including EREG, S100P, cyclooxygenase (COX)-2, phospho‑Akt (p-Akt), matrix metalloproteinase (MMP)-1, MMP-2, MMP-3, MMP-9, MMP-13 and MMP-14 were found following gene screening in SACC-83 and SACC-LM cells. Compared with SACC-83, SACC-LM presents higher expression of COX-2, S100P and lower expression of MMP-2, p-Akt, which could be candidates for identifying the homologous pair cell lines.

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