Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study

  • Authors:
    • Bruna Victorasso Jardim
    • Marina Gobbe Moschetta
    • Camila Leonel
    • Gabriela Bottaro Gelaleti
    • Vitor Rafael Regiani
    • Lívia Carvalho Ferreira
    • Juliana Ramos Lopes
    • Debora Ap. Pires de Campos Zuccari
  • View Affiliations

  • Published online on: June 13, 2013     https://doi.org/10.3892/or.2013.2540
  • Pages: 1119-1128
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Abstract

The use of prognostic markers for breast cancer allows therapeutic strategies to be defined more efficiently. The expression of glutathione (GSH) and glutathione peroxidase (GPX) in tumor cells has been evaluated as a predictor of prognosis and response to cytotoxic treatments. Its immunoexpression was assessed in 63 women diagnosed with invasive ductal carcinoma in a retrospective study. The results showed that high GSH expression was associated with tumors negative for the estrogen receptor (ER) (P<0.05), and GPX expression was associated with tumors negative for the progesterone receptor (PR) and patient mortality. Focusing on the 37 patients who received adjuvant chemotherapy/radiotherapy (Group I), high expression of GPX was associated with a high rate of patient mortality (P<0.05). The 19 patients who received only adjuvant chemotherapy (Group II) showed high expression of GSH in relation to metastasis (P<0.05). In addition, high levels of GPX expression were significantly associated with a shorter overall survival (P<0.05). To confirm this, the expression of precursor genes of GSH [glutamate cysteine ligase (GCLC) and glutathione synthetase (GSS)] and the GPX gene was analyzed using quantitative PCR in cultured neoplastic mammary cells treated with doxorubicin. Doxorubicin treatment was able to eliminate tumor cells without alterations in the gene expression of GSS, but led to underexpression of the GCLC and GPX genes. Our results suggest that high levels of GPX may be related to the development of resistance to chemotherapy in these tumors, response to treatment and the clinical course of the breast cancer patients.
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September 2013
Volume 30 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Jardim BV, Moschetta MG, Leonel C, Gelaleti GB, Regiani VR, Ferreira LC, Lopes JR and de Campos Zuccari DA: Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study. Oncol Rep 30: 1119-1128, 2013.
APA
Jardim, B.V., Moschetta, M.G., Leonel, C., Gelaleti, G.B., Regiani, V.R., Ferreira, L.C. ... de Campos Zuccari, D.A. (2013). Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study. Oncology Reports, 30, 1119-1128. https://doi.org/10.3892/or.2013.2540
MLA
Jardim, B. V., Moschetta, M. G., Leonel, C., Gelaleti, G. B., Regiani, V. R., Ferreira, L. C., Lopes, J. R., de Campos Zuccari, D. A."Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study". Oncology Reports 30.3 (2013): 1119-1128.
Chicago
Jardim, B. V., Moschetta, M. G., Leonel, C., Gelaleti, G. B., Regiani, V. R., Ferreira, L. C., Lopes, J. R., de Campos Zuccari, D. A."Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study". Oncology Reports 30, no. 3 (2013): 1119-1128. https://doi.org/10.3892/or.2013.2540