Thymidine kinase/ganciclovir and cytosine deaminase/5-fluorocytosine suicide gene therapy-induced cell apoptosis in breast cancer cells

  • Authors:
    • H. Kong
    • L. Tao
    • K. Qi
    • Y. Wang
    • Q. Li
    • J. Du
    • Z. Huang
  • View Affiliations

  • Published online on: June 21, 2013     https://doi.org/10.3892/or.2013.2562
  • Pages: 1209-1214
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Abstract

The present study was conducted to explore the efficacy of suicide gene therapy with thymidine kinase (TK) in combination with cytosine deaminase (CD) for breast cancer. The expression of CD/TK was detected in the infected cells by RT-PCR. The killing effect on MCF-7 cells following treatment was analyzed by MTT assay. The morphological characteristics of the cells were observed by electron microscopy, and the distribution of the cell cycle was analyzed by flow cytometry. Caspase‑3 and -8 activities were detected by absorption spectrometry. Cytotoxic assays showed that cells transfected with CD/TK became more sensitive to the prodrugs. Morphological features characteristic of apoptosis were noted in the MCF‑7 cells via electron microscopy. The experimental data showed that the proportion of MCF-7 cells during the different phases of the cell cycle varied significantly following treatment with the prodrugs. The activity of caspase‑3 gradually increased following treatment with increasing concentrations of the prodrugs. We conclude that the TK/ganciclovir and CD/5-fluorocytosine suicide gene system used here induces apoptosis in breast cancer cells, and provides a promising treatment modality for breast cancer.
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September 2013
Volume 30 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kong H, Tao L, Qi K, Wang Y, Li Q, Du J and Huang Z: Thymidine kinase/ganciclovir and cytosine deaminase/5-fluorocytosine suicide gene therapy-induced cell apoptosis in breast cancer cells. Oncol Rep 30: 1209-1214, 2013.
APA
Kong, H., Tao, L., Qi, K., Wang, Y., Li, Q., Du, J., & Huang, Z. (2013). Thymidine kinase/ganciclovir and cytosine deaminase/5-fluorocytosine suicide gene therapy-induced cell apoptosis in breast cancer cells. Oncology Reports, 30, 1209-1214. https://doi.org/10.3892/or.2013.2562
MLA
Kong, H., Tao, L., Qi, K., Wang, Y., Li, Q., Du, J., Huang, Z."Thymidine kinase/ganciclovir and cytosine deaminase/5-fluorocytosine suicide gene therapy-induced cell apoptosis in breast cancer cells". Oncology Reports 30.3 (2013): 1209-1214.
Chicago
Kong, H., Tao, L., Qi, K., Wang, Y., Li, Q., Du, J., Huang, Z."Thymidine kinase/ganciclovir and cytosine deaminase/5-fluorocytosine suicide gene therapy-induced cell apoptosis in breast cancer cells". Oncology Reports 30, no. 3 (2013): 1209-1214. https://doi.org/10.3892/or.2013.2562