Advantages of the rapid double-staining method for intraoperative detection of micrometastasis in sentinel lymph nodes

  • Authors:
    • Toshihiko Ojima
    • Shinichi Kinami
    • Keishi Nakamura
    • Katsunobu Oyama
    • Masashi Inokuchi
    • Hideto Fujita
    • Itasu Ninomiya
    • Sachio Fushida
    • Takashi Fujimura
    • Seiko Kitamura
    • Shinichi Harada
    • Tetsuo Ohta
  • View Affiliations

  • Published online on: July 4, 2013     https://doi.org/10.3892/or.2013.2591
  • Pages: 1067-1072
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Abstract

For rapid intraoperative diagnosis of lymph node micrometastasis, we refined the rapid immunohistochemistry method by combining anti-cytokeratin antibody-labeled nanocrystal beads with rapid hematoxylin and eosin (H&E) staining on the same section, referred to as the rapid double staining (RDS) technique. Two frozen-section slices each were obtained from 372 lymph nodes of 100 breast cancer patients. We performed RDS for 1 slide and rapid H&E staining for the other. The results were compared with the corresponding final pathological data obtained from the permanent specimens. For specimens from patients with pN1(mi) as determined by final pathological examination, the false‑negative rate was 33.3% for rapid H&E staining and 16.7% for RDS. For specimens from patients with pN0(i+) as determined by final pathological examination, the false‑negative rate was 80% for rapid H&E staining and 0% for RDS. These results indicate that RDS is superior to conventional rapid H&E staining for intraoperative diagnosis of nodal micrometastasis and isolated tumor cells.
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September 2013
Volume 30 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ojima T, Kinami S, Nakamura K, Oyama K, Inokuchi M, Fujita H, Ninomiya I, Fushida S, Fujimura T, Kitamura S, Kitamura S, et al: Advantages of the rapid double-staining method for intraoperative detection of micrometastasis in sentinel lymph nodes. Oncol Rep 30: 1067-1072, 2013.
APA
Ojima, T., Kinami, S., Nakamura, K., Oyama, K., Inokuchi, M., Fujita, H. ... Ohta, T. (2013). Advantages of the rapid double-staining method for intraoperative detection of micrometastasis in sentinel lymph nodes. Oncology Reports, 30, 1067-1072. https://doi.org/10.3892/or.2013.2591
MLA
Ojima, T., Kinami, S., Nakamura, K., Oyama, K., Inokuchi, M., Fujita, H., Ninomiya, I., Fushida, S., Fujimura, T., Kitamura, S., Harada, S., Ohta, T."Advantages of the rapid double-staining method for intraoperative detection of micrometastasis in sentinel lymph nodes". Oncology Reports 30.3 (2013): 1067-1072.
Chicago
Ojima, T., Kinami, S., Nakamura, K., Oyama, K., Inokuchi, M., Fujita, H., Ninomiya, I., Fushida, S., Fujimura, T., Kitamura, S., Harada, S., Ohta, T."Advantages of the rapid double-staining method for intraoperative detection of micrometastasis in sentinel lymph nodes". Oncology Reports 30, no. 3 (2013): 1067-1072. https://doi.org/10.3892/or.2013.2591