NDRG2 positively regulates E-cadherin expression and prolongs overall survival in colon cancer patients

  • Authors:
    • Young-Jun Kim
    • Ho-Bum Kang
    • Hyun Sun Yim
    • Joo Heon Kim
    • Jae Wha Kim
  • View Affiliations

  • Published online on: July 29, 2013     https://doi.org/10.3892/or.2013.2642
  • Pages: 1890-1898
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Abstract

To discover the molecular mechanism of N-Myc downstream-regulated gene 2 (NDRG2), a newly found differentiation-related tumor suppressor, the relationships between NDRG2 and E-cadherin were investigated in tumor cells and tissues. Positive correlations between the expression of E-cadherin and NDRG2 were shown in several colon cancer cell lines as well as in colon cancer tissues. According to the transcription assays using a reporter plasmid containing E-cadherin promoter region (-368~+51), NDRG2 introduction into colon cancer cell lines induced upregulation of E-cadherin promoter activity and its transcription. On the contrary, inhibition of NDRG2 expression by siRNA treatment caused the decrease of E-cadherin transcription. Snail, a zinc-finger transcriptional repressor, was shown to be a mediator of NDRG2-regulated E-cadherin expression. The enhancement of glycogen synthase kinase 3β (GSK-3β) activity by NDRG2 overexpression caused proteasomal degradation of Snail transcription factor followed by transcriptional de-repression of E-cadherin. We also found that NDRG2 could mediate cell density-regulated E-cadherin expression. The increase of NDRG2 expression with cell density preceded E-cadherin expression, and the regulation of Snail activity by GSK-3β was also related to this process.
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October 2013
Volume 30 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Kim Y, Kang H, Yim HS, Kim JH and Kim JW: NDRG2 positively regulates E-cadherin expression and prolongs overall survival in colon cancer patients. Oncol Rep 30: 1890-1898, 2013.
APA
Kim, Y., Kang, H., Yim, H.S., Kim, J.H., & Kim, J.W. (2013). NDRG2 positively regulates E-cadherin expression and prolongs overall survival in colon cancer patients. Oncology Reports, 30, 1890-1898. https://doi.org/10.3892/or.2013.2642
MLA
Kim, Y., Kang, H., Yim, H. S., Kim, J. H., Kim, J. W."NDRG2 positively regulates E-cadherin expression and prolongs overall survival in colon cancer patients". Oncology Reports 30.4 (2013): 1890-1898.
Chicago
Kim, Y., Kang, H., Yim, H. S., Kim, J. H., Kim, J. W."NDRG2 positively regulates E-cadherin expression and prolongs overall survival in colon cancer patients". Oncology Reports 30, no. 4 (2013): 1890-1898. https://doi.org/10.3892/or.2013.2642