Flavonoids from Citrus unshiu Marc. inhibit cancer cell adhesion to endothelial cells by selective inhibition of VCAM-1

  • Authors:
    • Hana Jin
    • Won Sup Lee
    • Jeong Won Yun
    • Ji Hyun Jung
    • Sang Mi Yi
    • Hye Jung Kim
    • Yung Hyun Choi
    • Gonsup Kim
    • Jin-Myung Jung
    • Chung Ho Ryu
    • Sung Chul Shin
    • Soon Chan Hong
  • View Affiliations

  • Published online on: August 30, 2013     https://doi.org/10.3892/or.2013.2711
  • Pages: 2336-2342
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Abstract

Citrus fruits have been used as edible fruit and a component of traditional medicine for various diseases including cancer since ancient times. Herein, we investigated the anticancer activity of flavonoids of Citrus unshiu Marc. (FCM) focusing on anti-metastatic effects. We prepared FCM and performed experiments using MDA-MB-231 human breast cancer cells. FCM inhibited TNF-induced cancer cell adhesion to human umbilical vein endothelial cells (HUVECs) without showing any toxicity. FCM inhibited the expression of VCAM-1, but not of ICAM-1, on MDA-MB-231 cells as well as HUVECs. FCM inhibited protein kinase C (PKC) phosphorylation, but not Akt phosphorylation. FCM also inhibited cancer cell invasion in a dose-dependent manner, but not MMP-9 expression. In conclusion, this study suggested that FCM inhibits TNF-induced cancer cell adhesion to HUVECs by inhibiting VCAM-1 through inhibition of PKC, providing evidence that FCM have anti-metastatic activity by inhibiting adhesion molecules and invasion on human breast cancer cells.
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November 2013
Volume 30 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Jin H, Lee WS, Yun JW, Jung JH, Yi SM, Kim HJ, Choi YH, Kim G, Jung J, Ryu CH, Ryu CH, et al: Flavonoids from Citrus unshiu Marc. inhibit cancer cell adhesion to endothelial cells by selective inhibition of VCAM-1. Oncol Rep 30: 2336-2342, 2013.
APA
Jin, H., Lee, W.S., Yun, J.W., Jung, J.H., Yi, S.M., Kim, H.J. ... Hong, S.C. (2013). Flavonoids from Citrus unshiu Marc. inhibit cancer cell adhesion to endothelial cells by selective inhibition of VCAM-1. Oncology Reports, 30, 2336-2342. https://doi.org/10.3892/or.2013.2711
MLA
Jin, H., Lee, W. S., Yun, J. W., Jung, J. H., Yi, S. M., Kim, H. J., Choi, Y. H., Kim, G., Jung, J., Ryu, C. H., Shin, S. C., Hong, S. C."Flavonoids from Citrus unshiu Marc. inhibit cancer cell adhesion to endothelial cells by selective inhibition of VCAM-1". Oncology Reports 30.5 (2013): 2336-2342.
Chicago
Jin, H., Lee, W. S., Yun, J. W., Jung, J. H., Yi, S. M., Kim, H. J., Choi, Y. H., Kim, G., Jung, J., Ryu, C. H., Shin, S. C., Hong, S. C."Flavonoids from Citrus unshiu Marc. inhibit cancer cell adhesion to endothelial cells by selective inhibition of VCAM-1". Oncology Reports 30, no. 5 (2013): 2336-2342. https://doi.org/10.3892/or.2013.2711