Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells

Wu,King-Chuen; Yang,Su-Tso; Hsu,Shu-Chun; Chiang,Jo-Hua; Hsia,Te-Chun; Yang,Jai-Sing; Liu,Kuo-Ching; Wu,Rick  Sai-Chuen; Chung,Jing-Gung

doi:10.3892/or.2013.2722

Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells

Authors:
- King-Chuen Wu
- Su-Tso Yang
- Shu-Chun Hsu
- Jo-Hua Chiang
- Te-Chun Hsia
- Jai-Sing Yang
- Kuo-Ching Liu
- Rick Sai-Chuen Wu
- Jing-Gung Chung
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Affiliations: Department of Anesthesiology, E-DA Hospital/I-Shou University, Kaohsiung 824, Taiwan, R.O.C., Department of Radiology, China Medical University Hospital, Taichung 404, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan, R.O.C., Department of Internal Medicine, China Medical University Hospital, Taichung 404, Taiwan, R.O.C., Department of Pharmacology, China Medical University, Taichung, Taiwan, R.O.C., Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Anesthesiology, China Medical University Hospital, Taichung 404, Taiwan, R.O.C.
Published online on: September 5, 2013 https://doi.org/10.3892/or.2013.2722
Pages: 2304-2310

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Abstract

Propofol is one of the most widely clinically used intravenous anesthetic, and it induces apoptosis in human and murine leukemia cell lines. Yet, whether propofol causes DNA damage and affects the mRNA expression of repair-associated genes in cancer cells remains undetermined. In the present study, we investigated the effects of propofol on DNA damage and associated mRNA gene expression in RAW264.7 cells. Comet assay and DNA gel electrophoresis were used to evaluate DNA damage in RAW264.7 cells and propofol-inhibited cell growth in vitro. The results revealed a longer DNA tail and DNA fragmentation. Real-time PCR assay was used to examine mRNA gene expression of DNA damage and DNA repair-associated genes. Following exposure to propofol for 48 h, a decrease in the mRNA expression of DNA-PK, BRCA1, MGMT and p53 was noted in the RAW264.7 cells. Results from the western blotting indicated that p53, MGMT, 14-3-3-σ, BRCA1 and MDC1 proteins were decreased while p-p53 and p-H2A.X(S140) were increased in the RAW264.7 cells following exposure to propofol. In conclusion, exposure to propofol caused DNA damage and inhibited mRNA expression and protein levels of repair-associated genes in RAW264.7 cells.

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