Downregulation of Hsp70 inhibits apoptosis induced by sialic acid-binding lectin (leczyme)

Tatsuta,Takeo; Hosono,Masahiro; Ogawa,Yukiko; Inage,Kyoko; Sugawara,Shigeki; Nitta,Kazuo

doi:10.3892/or.2013.2814

Downregulation of Hsp70 inhibits apoptosis induced by sialic acid-binding lectin (leczyme)

Authors:
- Takeo Tatsuta
- Masahiro Hosono
- Yukiko Ogawa
- Kyoko Inage
- Shigeki Sugawara
- Kazuo Nitta
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Affiliations: Division of Cell Recognition Study, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Sendai 981-8558, Japan, Divisions of Functional Morphology and Microbiology, Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, Sasebo, Nagasaki 859-3298, Japan
Published online on: October 24, 2013 https://doi.org/10.3892/or.2013.2814
Pages: 13-18
Copyright: © Tatsuta et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Heat shock proteins (Hsps) are molecular chaperones that maintain homeostasis of organisms. In regards to the Hsps, many studies have investigated the structure, expression, localization and functions of Hsp70 and Hsc70 including expression in the glycosphingolipid-enriched microdomain (GEM) on the cell surface and involvement in cell death. Sialic acid-binding lectin (SBL) isolated from oocytes of Rana catesbeiana is a multifunctional protein which has lectin activity, ribonuclease activity and antitumor activity. SBL has potential as a new type of anticancer drug, since it causes cancer-selective induction of apoptosis by multiple signaling pathways in which RNA is its target; and the participation of the mitochondrial pathway and the endoplasmic reticulum (ER) stress-mediated pathway has been suggested. It has also been suggested that receptor(s) for SBL (SBLR) may exist in the GEM on the cell surface. In the present study, we studied the possible involvement of Hsp70 and Hsc70 in SBL-induced apoptosis. We showed that Hsp70 and Hsc70 were expressed on the P388 cell surface similar to SBLR, and their distribution in cells dramatically changed immediately prior to the execution of apoptosis following stimulation of SBL. Functional study of Hsp70 revealed that decreased expression of Hsp70 diminished the apoptosis induced by SBL. It is suggested that Hsp70 participates in the antitumor effect of SBL.

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