Hydroxytyrosol inhibits cholangiocarcinoma tumor growth: An in vivo and in vitro study

  • Authors:
    • Shuai Li
    • Zhiyang Han
    • Yong Ma
    • Ruipeng Song
    • Tiemin Pei
    • Tongsen Zheng
    • Jiabei Wang
    • Dongsheng Xu
    • Xiang Fang
    • Hongchi Jiang
    • Lianxin Liu
  • View Affiliations

  • Published online on: November 14, 2013     https://doi.org/10.3892/or.2013.2853
  • Pages: 145-152
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cholangiocarcinoma (CCA) is a type of digestive tumor that is associated with a high rate of mortality due to the difficulty of early diagnosis and the resistance of this tumor type to chemotherapy. Hydroxytyrosol (HT), which is derived from virgin olive oil (VOO), has recently been reported to inhibit the proliferation of various types of human cancer cells. In the present study, we investigated the effect of HT on CCA. The antiproliferative and proapoptotic effects of HT on CCA were evaluated in the human CCA cell lines TFK-1 and KMBC and the human gallbladder cancer cell line GBS-SD. We also assessed this effect in vivo. We found that 75 µM HT inhibited the proliferation of the TFK-1, KMBC and GBS-SD cell lines. However, 200 µM HT treatment did not affect the proliferation of the human bile duct cell line HIBEpiC. More importantly, HT (250 and 500 mg/kg/day) markedly inhibited the growth of CCA xenografts in mice. G2/M phase cell cycle arrest and apoptosis were observed using flow cytometry and western blotting, and we also noted a time- and dose-dependent inhibition of phospho-ERK, with no changes in total-ERK, during treatment with HT. The present study showed that HT induces cell cycle arrest and apoptosis in vitro and in vivo. These data suggest that HT, which possesses excellent biocompatibility and few side-effects, could be developed as a novel agent against CCA.
View Figures
View References

Related Articles

Journal Cover

2014-January
Volume 31 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Han Z, Ma Y, Song R, Pei T, Zheng T, Wang J, Xu D, Fang X, Jiang H, Jiang H, et al: Hydroxytyrosol inhibits cholangiocarcinoma tumor growth: An in vivo and in vitro study. Oncol Rep 31: 145-152, 2014.
APA
Li, S., Han, Z., Ma, Y., Song, R., Pei, T., Zheng, T. ... Liu, L. (2014). Hydroxytyrosol inhibits cholangiocarcinoma tumor growth: An in vivo and in vitro study. Oncology Reports, 31, 145-152. https://doi.org/10.3892/or.2013.2853
MLA
Li, S., Han, Z., Ma, Y., Song, R., Pei, T., Zheng, T., Wang, J., Xu, D., Fang, X., Jiang, H., Liu, L."Hydroxytyrosol inhibits cholangiocarcinoma tumor growth: An in vivo and in vitro study". Oncology Reports 31.1 (2014): 145-152.
Chicago
Li, S., Han, Z., Ma, Y., Song, R., Pei, T., Zheng, T., Wang, J., Xu, D., Fang, X., Jiang, H., Liu, L."Hydroxytyrosol inhibits cholangiocarcinoma tumor growth: An in vivo and in vitro study". Oncology Reports 31, no. 1 (2014): 145-152. https://doi.org/10.3892/or.2013.2853