Inhibition of CD73 stimulates the migration and invasion of B16F10 melanoma cells in vitro, but results in impaired angiogenesis and reduced melanoma growth in vivo

Koszałka,Patrycja; Pryszlak,Anna; Gołuńska,Monika; Kolasa,Justyna; Stasiłojć,Grzegorz; Składanowski,Andrzej C.; Bigda,Jacek J.

doi:10.3892/or.2013.2883

Inhibition of CD73 stimulates the migration and invasion of B16F10 melanoma cells in vitro, but results in impaired angiogenesis and reduced melanoma growth in vivo

Authors:
- Patrycja Koszałka
- Anna Pryszlak
- Monika Gołuńska
- Justyna Kolasa
- Grzegorz Stasiłojć
- Andrzej C. Składanowski
- Jacek J. Bigda
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Affiliations: Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG‑MUG, Medical University of Gdańsk, 80-211 Gdańsk, Poland
Published online on: November 29, 2013 https://doi.org/10.3892/or.2013.2883
Pages: 819-827

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Abstract

The role of ecto-5'-nucleotidase (CD73), an enzyme providing interstitial adenosine, was investigated in B16F10 melanoma progression. Chemical inhibition of CD73 decreased adherence of cells to extracellular matrix proteins in vitro and led to enhanced migration and invasion. Both processes were reversed by adenosine receptor agonists. In CD73‑deficient mice, tumor growth was decreased in comparison with that of wild-type animals. Additionally, the vasculature of CD73-inhibited tumors was impaired and neoangiogenesis in Matrigel plugs was reduced. It is, therefore, proposed that although CD73 shows anti-invasive and antimigratory function in B16F10 melanoma cells, its proangiogenic action is prevalent in vivo and may contribute to increased tumor growth.

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