The role of CD44+/CD24-/low biomarker for screening, diagnosis and monitoring of breast cancer

  • Authors:
    • Rosa Camerlingo
    • Giuseppe Andrea Ferraro
    • Francesco De Francesco
    • Maurizio Romano
    • Gianfranco Nicoletti
    • Maurizio Di Bonito
    • Massimo Rinaldo
    • Francesco D'Andrea
    • Giuseppe Pirozzi
  • View Affiliations

  • Published online on: December 23, 2013     https://doi.org/10.3892/or.2013.2943
  • Pages: 1127-1132
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Abstract

Cancer stem cells (CSCs) have been defined as ‘a cell within a tumor that possesses the capacity to self-renew and to cause the heterogeneous lineages of cancer cells that comprise the tumor’. The CSC hypothesis postulates that a small subpopulation of cancer cells drives tumor initiation, growth and metastasis. CSCs have been isolated from breast cancer using CD44+/CD24-/low phenotype. The purpose of the present study was to evaluate the expression of CD44+/CD24-/low in two diverse breast carcinomas (ductal and lobular), and to determine the correlation between expression of CD44+/CD24-/low, and clinicopathological characteristics starting from human fresh breast cancer specimens. We analyzed specimens from 57 patients using CD44 and CD24 markers by flow cytometry and immunohistochemistry and correlated the CD44+/CD24-/low phenotype with clinicopathological characteristics. Moreover, mammosphere formation was tested. In all specimens tested, CD44+/CD24-/low phenotype was detectable with mean percentage of 4.73% as confirmed also by immunohistochemical analyses. A significant statistical association was found among these phenotypic groups and age, grade G3, estrogen and progesterone receptor, Ki-67 as well as lymph node metastasis. No correlation was found for histological type. In conclusion, our data showed that CD44+/CD24-/low phenotype was found at a high frequency in tumors pT2, G3, pN3, positive for Ki-67, and negative for estrogen and progesterone receptors highlighting the hypothesis that CD44+/CD24-/low profile correlates with the more aggressive clinical-pathological features of the disease.
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2014-March
Volume 31 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Camerlingo R, Ferraro GA, De Francesco F, Romano M, Nicoletti G, Di Bonito M, Rinaldo M, D'Andrea F and Pirozzi G: The role of CD44+/CD24-/low biomarker for screening, diagnosis and monitoring of breast cancer. Oncol Rep 31: 1127-1132, 2014.
APA
Camerlingo, R., Ferraro, G.A., De Francesco, F., Romano, M., Nicoletti, G., Di Bonito, M. ... Pirozzi, G. (2014). The role of CD44+/CD24-/low biomarker for screening, diagnosis and monitoring of breast cancer. Oncology Reports, 31, 1127-1132. https://doi.org/10.3892/or.2013.2943
MLA
Camerlingo, R., Ferraro, G. A., De Francesco, F., Romano, M., Nicoletti, G., Di Bonito, M., Rinaldo, M., D'Andrea, F., Pirozzi, G."The role of CD44+/CD24-/low biomarker for screening, diagnosis and monitoring of breast cancer". Oncology Reports 31.3 (2014): 1127-1132.
Chicago
Camerlingo, R., Ferraro, G. A., De Francesco, F., Romano, M., Nicoletti, G., Di Bonito, M., Rinaldo, M., D'Andrea, F., Pirozzi, G."The role of CD44+/CD24-/low biomarker for screening, diagnosis and monitoring of breast cancer". Oncology Reports 31, no. 3 (2014): 1127-1132. https://doi.org/10.3892/or.2013.2943