MicroRNA-100 regulates SW620 colorectal cancer cell proliferation and invasion by targeting RAP1B

Peng,Hui; Luo,Jun; Hao,Hu; Hu,Jun; Xie,Shang-Kui; Ren,Donglin; Rao,Benqiang

doi:10.3892/or.2014.3075

MicroRNA-100 regulates SW620 colorectal cancer cell proliferation and invasion by targeting RAP1B

Authors:
- Hui Peng
- Jun Luo
- Hu Hao
- Jun Hu
- Shang-Kui Xie
- Donglin Ren
- Benqiang Rao
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Affiliations: Department of Colorectal and Anal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China, Department of Pediatrics, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China, Guangdong Gastrointestinal Institute, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, P.R. China, Department of Gastrointestinal Anal Surgery and Institute of Gastroenterology, The Third Affiliated Hospital of Nanchang University, Nanchang 330008, P.R. China
Published online on: March 11, 2014 https://doi.org/10.3892/or.2014.3075
Pages: 2055-2062

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Abstract

MicroRNAs (miRNAs) have been demonstrated to play important roles in tumorigenesis of human cancer. Fewer studies have explored the roles of miR-100 on human colorectal cancer cell proliferation and invasion. In this study, we utilized real-time PCR to verify whether miR-100 was downregulated in human colorectal cancer tissues compared with matched adjacent normal tissues. Functional studies demonstrated that ectopic expression of miR-100 inhabits cell growth and invasion and induce apoptosis, whereas knockdown of miR-100 yielded the reverse phenotype. Mechanistic studies reveal that miR-100 repressed the activity of a reporter gene fused to the 3'-untranslated region (3'-UTR) of RAP1B, whereas miR-100 silencing upregulated the expression of the reporter gene. Furthermore, we also detected that RAP1B mRNA was inversely expressed with miR-100 in colorectal cancer tissues. These data indicate that the miR-100 plays a tumor suppressor role by regulating colorectal cancer cell growth and invasion phenotype, and could serve as a potential maker for colorectal cancer therapy.

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