MicroRNA-100 regulates SW620 colorectal cancer cell proliferation and invasion by targeting RAP1B

  • Authors:
    • Hui Peng
    • Jun Luo
    • Hu Hao
    • Jun Hu
    • Shang-Kui Xie
    • Donglin Ren
    • Benqiang Rao
  • View Affiliations

  • Published online on: March 11, 2014     https://doi.org/10.3892/or.2014.3075
  • Pages: 2055-2062
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Abstract

MicroRNAs (miRNAs) have been demonstrated to play important roles in tumorigenesis of human cancer. Fewer studies have explored the roles of miR-100 on human colorectal cancer cell proliferation and invasion. In this study, we utilized real-time PCR to verify whether miR-100 was downregulated in human colorectal cancer tissues compared with matched adjacent normal tissues. Functional studies demonstrated that ectopic expression of miR-100 inhabits cell growth and invasion and induce apoptosis, whereas knockdown of miR-100 yielded the reverse phenotype. Mechanistic studies reveal that miR-100 repressed the activity of a reporter gene fused to the 3'-untranslated region (3'-UTR) of RAP1B, whereas miR-100 silencing upregulated the expression of the reporter gene. Furthermore, we also detected that RAP1B mRNA was inversely expressed with miR-100 in colorectal cancer tissues. These data indicate that the miR-100 plays a tumor suppressor role by regulating colorectal cancer cell growth and invasion phenotype, and could serve as a potential maker for colorectal cancer therapy.
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May-2014
Volume 31 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Peng H, Luo J, Hao H, Hu J, Xie S, Ren D and Rao B: MicroRNA-100 regulates SW620 colorectal cancer cell proliferation and invasion by targeting RAP1B. Oncol Rep 31: 2055-2062, 2014.
APA
Peng, H., Luo, J., Hao, H., Hu, J., Xie, S., Ren, D., & Rao, B. (2014). MicroRNA-100 regulates SW620 colorectal cancer cell proliferation and invasion by targeting RAP1B. Oncology Reports, 31, 2055-2062. https://doi.org/10.3892/or.2014.3075
MLA
Peng, H., Luo, J., Hao, H., Hu, J., Xie, S., Ren, D., Rao, B."MicroRNA-100 regulates SW620 colorectal cancer cell proliferation and invasion by targeting RAP1B". Oncology Reports 31.5 (2014): 2055-2062.
Chicago
Peng, H., Luo, J., Hao, H., Hu, J., Xie, S., Ren, D., Rao, B."MicroRNA-100 regulates SW620 colorectal cancer cell proliferation and invasion by targeting RAP1B". Oncology Reports 31, no. 5 (2014): 2055-2062. https://doi.org/10.3892/or.2014.3075